إنتشار جينات hsp65 و mpt64 في بكتيريا Mycobacterium tuberculosis في محافظة كربلاء

Prevalence of hsp65 and mpt64 genes in bacteria Mycobacterium tuberculosis in Karbala province

Abstract

The study was conducted to illuminate on the relationship of the two genes hsp65 and mpt64 and the incidence of tuberculosis and its prevalence in the Karbala governorate, where the study included 120 samples from the total auditors. Staining by Acid-fast stain and microscopic examination. The number of samples taken was 120 samples, including (50 positive samples, 41.7%) and negative cases (70 samples, 58.3%) The samples were classified according to the injury sample into Chronic, Acute, Recurrent; The results of the relationship of gender with the type of environment (urban, rural) showed that there were no significant differences between the injured males of urban and rural residents and between females of urban and rural residents, as well as the relationship of smoking with the sample environment (urban, rural) showed no significant differences between the injured Smokers and non-smokers in urban and rural areas. The results of the study of the relationship of gender with smoking showed that there were significant differences between male and female smokers, as males recorded 28 samples with a percentage of 100% compared to females with zero samples and 0%, and non-smoking females recorded a significant increase with 16 samples with a percentage of 72.7% compared to males with 6 samples and a percentage of 27.3 Male smokers recorded a significant increase with 28 samples, or 82.4%, compared to non-smokers with 6 samples, or 17.6%, and female non-smokers recorded a significant increase with 16 samples, with a percentage of 100%, compared to zero smokers, i.e. 0%; And the relationship of gender with the categories of sputum smears for smear-positive sputum patients was recorded, with no significant differences between the group +1 for males and females, while the group +2 recorded a significant increase in males with 12 samples at a rate of 66.7% compared with females with 6 samples at a rate of 33.3%, while Category +3 recorded a significant increase in males with 12 samples at a percentage of 100% compared to females who recorded zero samples at a percentage of 0%, while the relationship of smoking with the affected groups was clarified, and category +3 recorded a significant increase in smokers with 12 samples at a rate of 42.9%, followed by category +2 with 10 samples. At a rate of 55.6%, then category +1 with 6 samples and a percentage of 30%, while the samples with the infected groups were non-smokers, the group +1 had a significant increase of 14 samples and 70.0%, followed by category +2 with 8 samples and at a rate of 44.4%, while the percentage of category +3 was zero A sample compared with the rest of the other groups. The results of the study indicated the relationship of the type of environment with the affected groups, as it indicated that there were significant differences between the group +1 in urban 12 samples with a percentage of 60% and rural with 8 samples at a percentage of 40%, while the category +2 in urban was 12 samples at a rate of 66.7 % compared to the countryside with 6 samples, with a percentage of 33% 3%, and for the +3 category in urban 12 samples at 100% compared to 0% in rural areas. The results were also observed when using specialized primers to detect primers of the hsp65 gene for the presence of a package resulting from the inflation process in the first, fourth and fifth paths: Recurrent, path The second and third: Acute, Sixth, Seventh and Eighth Paths: Chronic, which shows the presence of a piece of DNA at the expected molecular weight of this gene corresponding to 195 base pairs. When using specialized primers to detect primers of the mpt64 gene, the presence of a bundle resulting from the amplification process in the first and fourth pathways Fifth: Recurrent, pathways two and three: Acute, pathways six, seven and eight: Chronic, which show the presence of a piece of DNA at the expected molecular weight of this gene corresponding to 771 base pairs; The results of the direct examination using Acid Fast stain showed 56 positive samples with a percentage of 50.9%, while the results using the PCR technique were positive in 50 samples and at a rate of 45.45%, where the sensitivity and specificity recorded 78%, and a confidence range ranging from 64.04-88.47% and 71.67%, and a confidence range ranging from 58.56-82.55% Straight.