االكشف االجزيئي البعض أنزيمات الكاربابنيميز في بكتريا Klebsiella pneumoniae

Molecular detection of some carbapenemases in Klebsiella pneumoniae isolated from different clinical sources

This study included a diagnosis of Klebsiella pneumoniae responsible for many infections after being isolated from different clinical sources such as blood, sputum, burns, wounds, the pharynx, the ear, urine, and vagina, and also the investigation of virulence factors these bacteria cross detecting the following genes: blaKPC, blaIMP, blaVIM, blaNDM, and blaOXA48, which encode for beta-lactam-degrading.
The results yielded in Sixty-eight isolates being obtained out of 275 samples that were collected from patients who were reviewed and inpatients in hospitals and outpatient clinics in the Holy Karbala Governorate, of both sexes and of different ages, for the period between the first of September and the end of December 2022. It was diagnosed through phenotypic examination on culture imedia blood agars and MacConkey agars, and by using Vitek-2 system.
The sensitivity of the bacterial isolates obtained in this study was conducted against 12 types of antibiotics that are used in the treatment of K. pneumoniae infections, which included Meropenem, Imipenem, Cefipeme, Cefotaxime, Ceftazidime, Gentamicin, Aztreonam, Levofloxacin, Ciprofloxacin, Trimethoprim-sulfamethoxazole, Amikacin, Piperacillin, and Tazabactam. The results showed that the highest rate of resistance was to antibiotics Imipenem 80% and followed by Cefotaxime 74%, while she was the lowest rate of resistance to Levofloxacin and Piperacillin/Tazabactam 37%. As was also evident from the results of this examination showed that 40 isolates were multi-resistant (multi-drug resistant).
This study also included a phenotypic examination of the multi-resistance was also carried out using the Combined Disc Synergy Test to detect the presence of metal beta-lactam enzymes that contain zinc ione and the result was positive for 33 isolates and negative for seven of them. The results of the molecular detection of our current study confirmed the presence of the gene in the highest percentage (60%) in K. pneumoniae bacteria isolates, followed by the blaOXA48 gene by 45%, then the
blaNDM gene by 30%, While it became clear that the blaIMP gene was present in a low percentage 12.5%, and the lowest percentage of the blaKPC gene by (2.5%) for 33 isolates out of 40 isolates, while it turns out that seven isolates devoid of any gene.