استخدام بوليمر الكيتوسان النانوي المطعم مع بعض الادوية في تثبيط خلايا سرطان الثدي نوع AMJ13

Using Chitosan Nano Polymer Grafted with Some Drugs in Inhibiting Breast Cancer Type AMJ13

Abstract

In this work, nano chitosan was prepared using the ultrasounic method to dissolve chitosan in an acetic acid aqueous solution. The nano chitosan was characterized by; (FT-IR, AFM, XRD and TEM) techniques. Several nano chitosan-drugs were synthesized using different drugs (Mefenamic acid, Ampicillin, Amoxicillin, Cephalexin, and Paracetemol) respectively, as shown in the following diagram.
The solubility properties of nano chitosan bonded with some drugs in different solvents were studied (H2O, Acetone, Ethanol, Hexane, DMSO, Chloroform and Toluene).
The release of drugs (Abs.) was measured by using UV-Vis a spectrophotometer at different pH (2.2, 6.0, 7.2 and 8.0) at a constant temperature 310 K as a function of time (hour and day).
The binding of drugs to the proteins of breast cancer cells was studied, the protein-bonding interactions were predicted, the active site of the target protein was prepared, as well as the study of the most effective binding energy of nano-chitosan drug and the type and length of nano-chitosan drugs bonds.
The effect of the drugs loaded on the nano chitosan-drugs (A1, A2, A3, A4, A5 and A6) in inhibiting the spread of breast cancer was measured. The results showed that the drugs had a different effect. The value was significantly decreased in A5 compared to other drugs. The effectiveness of drugs loaded on the nano chitosan in preventing the spread of breast cancer was in the following order:
A5 > A3 > A2> A6 > A4> A1