التأثير العلاجي والوقائي لأوراق نبات القريص ضد استحداث الفشل الكلوي المزمن وفقر الدم المتزامن في ذكور الجرذان

Protective and therapeutic effect of Urtica dioica Leaves against induce chronic renal failure and coincident anemia in male rats (Rattus albicans)

Abstract

This study is carried out to evaluate some coagulation factors, biochemical factors, histological changes of liver, kidney and bone marrow in adult male rats that caused chronic renal failure and the possible protective role of the treatment effect of Urtica dioica leaves on anemia induced by chronic renal failure.
Forty-five male rats were used in the study, divided randomly into five groups (9 mice per group) for 7 weeks, the first group (GI) control was with dimethyl sulfoxide (DMSO) by interaperitoneally for 4 weeks, the second group (GII) is the negative control was administrated Urtica Dioica leaves powder at given 4% mixed with diet. for 4 weeks, the third group (GIII) was administrated adenine dissolved by dimethyl sulfoxide (DMSO) interaperitoneally at dose 100 mg/kg.bw for 4 weeks for induction of renal failure, the fourth group (GIV) adenine dissolved by dimethyl sulfoxide (DMSO) was administrated interaperitoneally at dose 100mg/kg.bw for 4 weeks for induction of renal failure and then given 4% Urtica dioica leaves powder for 3 weeks mixed with diet, and the fifth group (GV) adenine dissolved by dimethyl sulfoxide (DMSO) was administrated interaperitoneally at dose 100mg/kg.bw for 4 weeks, and then given 4% Urtica dioica leaves powder mixed with diet at the same time. The results showed there were a significant elevation (p≤0.01) in the serum urea, serum creatinine, free erythrocyte protoprophyrine (FEP), white blood cells count (WBCs), serum ferritin and serum hepcidin in (GIII) adenine treated group and statistically significant decrease (p≤0.01) in red blood cells count, packed cell volume PCV, platelets, serum iron and hemoglobin (Hb) in adenine group (GIII) in comparison with the other groups, after Urtica dioica leaves administration we observe that there were a significant reduction in serum urea, creatinine, ferritin, FEP, hepcidin and WBCs in (GIV) treated group and statistically significant (p≤0.01) increase in RBC count, PVC, PLT, Fe and Hb in forth group (GIV) in comparing to (GIII) group.
Histological changes in kidney, liver and bone marrow demonstrate that adenine treated group was damaged atrophied and degeneration especially the renal tubules that have the adenine crystalline precipitation in add to inflammatory infiltrate of inflammatory in comparing to control group however after Urtica dioica leaves administration the tissues are able minimize the inflammatory condition but not fully recovered. This study explains the induction of renal failure by adenine and how can chronic renal failure results in anemia also explains how can Urtica dioica leaves powder works on improvement of anemia.