التأثير الوقائي للمستخلص الكحولي لعشبة تريبولوس تيريستريس ضد الخلل الوظيفي في الكبد والخصية المستحدث بواسطة السيترابين في ذكور الجرذان

Protective effects of alcoholic extract of Tribulus terrestris against Cytarabine induced hepatic and testicular dysfunction in male rats

Summary
The present study investigated the possible therapeutic protective, the efficacy as an antioxidant, and the protective effects against chemically induced reproductive dysfunction on spermatogenesis damage. The cytotoxic effects of numerous cancer treatments can lead to various general adverse consequences and impairments in spermatogenesis. Therefore, it remains an important concern that need further improvement. Cytarabine (Ara C), a widely used chemotherapy drug for hematological malignancies, exerts detrimental effects on male reproductive development and function by suppressing germ cell proliferation and inducing apoptosis. Twenty mature male rats will be divided into four groups. Each group contains 5 rats. The first group is a control group, the second group i.p ingection with Ara-C (25 mg/kg BW) and the third group is oraly with T. Tribulus terrestris extract (250 mg/kg) and fourth group is dosed with Ara-C (25 mg/kg BW) intraperitoneally + T. terrestris extract (250 mg/kg bw) orally for 28 days. The HPLC examination of the dry extract of TTE revealed the presence of a variety phenolic compounds. Our work aimed to investigate TTE protective function against Ara C-induced reproductive impairment in male adult rats. Ara C caused dose-dependent increase in serum liver enzymes activities (AST, ALT and ALP); elevated oxidative stress; impeded steroidogenesis (lower testosterone, follicle-stimulating hormone, luteinizing hormone. Furthermore, immediate exposure to Ara-C resulted in a decrease in 17 β-HSD activity, could be linked to the decrease in testosterone concentration in the blood, the reported histological abnormalities in the testes, and the quality of the semen, and raised caspase concentration as compared with other groups.

In conclusion Ara-C that revealed detectable spermatogenesis degeneration and causes testicular damage, and T. terrestris works to reduce cytarabine damage. The current investigation emphasized the importance of TTE in improving the changes caused by Ara-C injection in the testes of rats, perhaps due to the presence of antioxidant compounds.