التاثير الوقائي لحامض الفا لايبوك على جهاز القلب الوعائي في ذكور الارانب التي تتعرض لغلوتامات الصوديوم الاحادية

Protective Effect of α Lipoic acid(ALA) on Cardiovascular System of Male Rabbits Exposed to Mono Sodium Glutamate (MSG)

The study was designed to investigate the protective role of Alpha Lipoic Acid
(ALA)against Monosodium glutamate (MSG) induced heart and aorta damage in
male rabbits . Some cardiac biomarkers were estimated in the serum, e.g.
(Endothelin 1,troponin I and NO) and some enzyme activity (ATPase and NO
synthase ) ,Minerals (K, Na ,Ca) ,some oxidant (ONOO and MDA) and antioxidant
(GSH) ,lipid profile (LDL-C,vLDL-C ,HDL-C ,TC, TAG)concentration ,
Electrocardiograph intervals (P, QRS and T) were monitored and histopathological
examination of heart and aorta tissues were performed.
Thirty two adult male rabbits were randomly divided into four equal
groups(8/group),The first group was intubated tap water and served as control
group (GI).Rabbits in the second group were intubated orally with 8mg/kg B.W
daily of MSG (GII),while animals of the third group were intubated with 60 mg/kg
B.W daily of AIA (GIII). Animals in the last group ( GIV ) received 8mg/kg B.W
of MSG and 60 mg/kg B.W daily for 10 weeks.
blood samples were collected by cardiac punctures technique at 10 weeks of
experimental study to evaluate the possibility of recovery of heart and aorta tissue
damage induced by MSG.
The result of our study indicates a significant (p<0.05)increase in the serum
levels of ONOO, MDA and bad lipid profile (VLDL-C,LDL-C,TC,TG) with a
significant increase in the serum level of Cardiac troponin I and Endothelin-1 and a
significant increase in ATP synthase activity in animal group received MSG and a
significant increase in [Na] hypernatremia with significant decrease of [K]
hypokalemia , in addition the results revealed a significant (p<0.05) increase in
the body weight and heart weight , while a significant decrease in the serum level
I
concentration of GSH, HDL-C and NO. The results of electrocardiogram records
the current study showed the prolongation in the waves ( P,QRS,T) and (ST,QT)
intervals in the GII group compared to control and GIII,GIV groups .
Oral intubation of 60mg/kg B.W daily of ALA in GIII and together with MSG
group GIV .The results showed an improvement recovery in heart and aorta
finding through a significant(p<0.05) decrease in the serum level of the ONOO,
MDA ,bad lipid profile (LDL-C,VLDL-C,TC,TG). It also showed a significant
increase(P<0.05) in the serum level concentration in the GSH and NO compared
to GI,GII groups .
Our result showed a significant (p<0.05)decrease in the serum Endothelin-1 and
Troponin-I and ATP synthase ,serum activity and increase in the serum level of
[K] and a significant decrease in the [Na] in GIII and GIV compared with
another groups .The recovery role of the ALA in the electrocardiograph in the GIII
and GIV showed a significant(p<0.05)decrease in the (P, QRS,T)waves and
(ST,QT) intervals compared to GII group.
The histopathological examination of heart and aorta in GII received MSG
,showed the heart appeared to have significant damage necrosis such as
inflammation and fibrosis and infiltration of inflammatory cell, hemorrhage and
congestion ,on the other hand thickening in aorta compared with other group and
study shown protective role of ALA in Heart and aorta in GIII.
In conclusion , our study was conducted the injuries effect of MSG ,it should be
avoided especially in heart and aorta disorder from foods containing excess MSG
which can be fortified with orally intubation of 60mg/kg B.W of ALA to
recover its adverse effects