التاثير الوقائي للكلوتاثايون في التركيب الفسلجي و النسجي لجهاز العصبي المركزي ضد الجهد التاكسدي في اناث الجرذان

The Effect of Glutathione in the Protection Of physiological and histological structure of Central Nerves System against oxidative stress in female rat

Abstract

This study is carried out to investigate the protective role of reduced Glutathione on the central nerves system damage induced by D-galactose overload in female rats.
Twenty-four adult female rats weight 200 gram were divided into four groups (6 in each group), the first group was injected with normal slain Intraperitoneal(IP) for forty (40) days and served as control group (G1), rats in the second group were injected 100 mg/kg B .W IP for 40 day of D-galactose (G2), rats of the third group (G3) were injected with Glutathione(GSH) Intraperitoneal(IP) with 100 mg/kg B .W for 40 day, the fourth group (G4) was injected with mix of D-galactose and Glutathione with 100 mg/kg B .W for each daily for 40 day . The experiment was carried out in the laboratories of the College of Veterinary Medicine, University of Karbala
Fasting blood samples were collected by cardiac punctures technique at forty (41) day of experimental for collecting the serum for measuring: beta amyloid, Acetylcholine Esterase(AChE),Caspase3,total anti-oxidant capacity(TAC), Malondialdehyde (MDA), Reduced Glutathione(GSH), catalase(CAT), Superoxide dismutase(SOD), glucose and lipid profile (total cholesterol (TC), triglyceride (TAG), serum concentration of high density lipoprotein- cholesterol (HDL-C), low Density lipoprotein- cholesterol (LDL-C), Very Low Density lipoprotein- cholesterol(VLDL-C)).Furthermore brain and spinal cord sample were collected for histological and immunohistochemical study.
The results showed a significant (p<0.01) increase in Serum beta amyloid concentration in G2 and G4 Compared with control and G3 groups, and there is a significant (p<0.05) increase in Serum Ache in group G2 when compared with control and G3 groups. Serum Casp3 shows a significant (p<0.01) increase in G2 treated group when compared with G1, G3 and G4 groups. Serum Glucose level show significant (p<0.01) increase in G2 when Compared to G1, G3 and G4.A significant (p<0.01) decrease in body Wight in G2 group when compared to G1, G3, G4 groups. Serum Total antioxidant capacity (TAO-C) showed a significant (p<0.05) decrease in G2 when Compared with G1, G3 and G4 groups. Serum Reduced Glutathione showed a significant (p<0.01) decrease in G2 when Compared with G1, G3 and G4 groups. Serum Melanoaldihide showed a significant (p<0.01) increase in G2 when Compared with G1, G3 and G4 groups. Serum catalase showed significant (p<0.01) decrease in G2 when Compared with G1, G3 and G4 groups. Serum Superoxide dismutase showed significant decrease (p<0.01) in G2 when Compared with G1, G3 and G4 groups. Serum Cholesterol showed significant (p<0.05) increase in G2 when Compared with G1, G3 and G4 groups. Serum triglycerides showed significant (p<0.05) increase in G2 when Compared with G1, G3 and G4 groups. Serum High-density lipoproteins-cholesterol showed significant (p<0.01) decrease in G2 when Compared with G1, G3 and G4 groups. Serum Low- density lipoproteins-cholesterol showed significant (p<0.01) increase in G2 when Compared with G1, G3 and G4 groups. Serum Very Low- density lipoproteins-cholesterol showed significant (p<0.05) increase in G2 when Compared with G1, G3 and G4 groups.
Histological change showed a chronic inflammation Neurodegeneration with beta amyloid deposition and TUNEL-positive in group that received 100 mg/kg.B.W of D-galactose, in another hand the combination group showed the ameliorate role of GSH in the neuro tissue.
The study concluded that D-galactose case oxidative stress to the central nerves system and glutathione plays a crucial role in physiological neruemetar , oxidant-antioxidant balance and apoptotic factors on the CNS of female rat