التحري عن سم الافلاتوكسين B1 لدى مرضى السكري النوع الثاني وتأثيره على وظائف الكلى والكبد

Investigation of Aflatoxin B1 in the Blood of Diabetes types 2 patients and its effects on Kidney and liver functions

Summary
This study was aimed to investigation of aflatoxin B1 in blood serum of patients with diabetes type_2 samples were collected from Al-Hussian hospital, Karbala province. From November 2021 till March 2022. The number was (86) samples; (42) samples were for patients with diabetes disease and (44) samples were for healthy people.
The results showed that 16 (38.1%) out 42 samples that collected from patients with diabetes type_2 contamination with aflatoxin B1, while 13 (29.5%) sample that collected from healthy persons were contamination with Aflatoxin B1 with no significant difference between them.
Also the amount of blood serum collected from females and males patients with diabetes type 2 and contamination with AFB1was 17 (19.7%) and 12 (13.9%) respectively.
The results illustrated the AFB1 concentration in blood serum of females and males patients with diabetes were 1.343 ng/ml and 0.684 ng/ml respectively with significant difference between them while the concentration of toxin in blood serum of females and males of healthy persons were 0.133 ng/ml and 0.135 ng/ml respectively with no significant difference between them.
Measurement coefficient (r) appearance there is correlation between gender and presence of AFB1 in blood serum (r=0.974 p value 0.324) it means the females more sensitive to AFB1 if compared with males groups. Males patients with diabetes and presence of AFB1 in blood serum of them lead to increased urea level to 42 mg/ml (abnormal level) compare 34 mg/dl in blood serum of males health. also creatinine level increased in blood serum of males patients with diabetes and borne AFB1 to 1.88 mg/dl.
also uric acid level raised to 8.3 mg/dl in blood serum of males patients with diabetes and borne AFB1 compare with its level in males healthy without AFB1 was 5.87 mg/dl on the other hand alkaline phosphate (ALP) increased to abnormal level in ( blood serum of patients male without Aflatoxin B1) group and (female patients without Aflatoxin B1) group reached to 145.8 U/L and 147.1 U/L respectively. Also alanine amino transferase (ALT) parameter raised to (18.15 and 15.08) U/L in blood serum of (males patients with diabetes and with Aflatoxin B1) group and (males patients with diabetes without Aflatoxin B1) group respectively.
The result of measurement of Aspartate aminotransferase (AST) in blood serum of males and females patients with diabetes type 2 without Aflatoxin B1 raised to 53.38 U/L and 49.82 U/L respectively.