التخليق الأخضر لجسيمات النحاس النانوية بأستخدام مستخلص أوراق الياس: التوصيف والتطبيقات

Green Synthesis of Copper Nanoparticles Using Myrtus communis Leaves Extract: Characterization and Applications

The biological synthesis of nanoparticles is being carried out by different organisms such as plants, bacteria, fungi, seaweeds, and microalgae. Plants extracts are considered one of the safest methods in green chemistry for synthesizing nanoparticles.
This study was designed to synthesize copper nanoparticles from safety substance (plant) and evaluate their biochemical and biological activity.
Different techniques were used to characterize copper nanoparticles. Ultraviolet-Visible absorption spectroscopy (UV-Vis) was used to examine the Surface Plasmon Resonance (SPR) of the nanoparticles in the range from 300 to 700 nm. The influential functional groups that can bio-reduce the copper ion Cu2+ were identified using Fourier Transform Infrared (FT-IR) spectroscopy. The crystal structure of copper nanoparticles was determined using X-ray diffraction (XRD), as evidenced by the peaks at 2θ values of 43.35, 50.50, and 74.21o. In transmission electron microscopy (TEM), the particles have spherical morphology with an average diameter of 35–75 nm, while scanning electron microscopy (SEM) reveals the sphere-like shape of Copper nanoparticles. The atomic force microscopy (AFM) analysis showed the size and the surface properties of biosynthesized nanoparticles and revealed an average size of 55 nm.
synthesized Copper nanoparticles in this study were examined as antioxidants and catalysis. The results showed that Copper nanoparrticles had superior radical scavenging activity when compared to an extract from Myrtus communis leaves. It is common to use 4-nitrophenol (4-NP) as a model reaction to evaluate synthesized nanomaterials’ catalytic properties, results show that CuNPs have better catalytic activity than extract at higher concentrations.

In this study, the efficacy of both CuNPs and extract against bacteria and their anti-inflammatory activity were tested. Antibacterial activity of the (CuNPs) and M. communis extract was evaluated against Gram-negative bacteria (Klebsiella pneumonia and Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus and Lactobacillus salivarins). In vitro, the anti‐inflammatory activity was evaluated for both extract and CuNPs using (albumin denaturation assay, membrane stabilization assay, and proteinase inhibitory activity) at different concentrations. The results of CuNPs exhibited a strong anti-inflammatory activity compared with standard drug (Aspirin).
In conclusion, M. communis is considered a good source for reducing copper salt into copper nanoparticles and the last have antioxidant and anti-inflammatory activity, besides they can be used as a catalyst.