الترابط بين أنزيم الداي ببتديل ببتيديز-4 وتعدد الاشكال الجيني rs1861978 ومستوى الانكرتين لمرضى السكري النوع الثاني في كربلاء المقدسة

Association between Dipeptidyl Peptidase-4 Polymorphism (rs1861978)and Incretin Level in Type 2 Diabetic Patients in Holy Kerbala

Abstract

Background
Type2 Diabetes Mellitus is associated with progressively increasing morbidity and
mortality across the globe. It is a complex multigenic disease with severe
consequences.
As several genes involved in Type2 Diabetes Mellitus regulations, Single Nucleotide
Polymorphism rs1861978 in dipeptidyl peptidase-4 has been suggested to be
associated with this disease.
On the other hand, Incretin hormone like Glucose-Dependent Insulinotropic
Polypeptide (GIP) in recent researches has been found to be linked to Type2 Diabetes
Mellitus in different circumstances.
Aim of the Study
This study aimed to investigate the possible association of single nucleotide
polymorphisms rs1861978 of the DPP4 gene in kerbala subjects with Type2 Diabetes
Mellitus and evaluate whether they had an effect on the serum levels of GIP levels.
Objectives
This study designed to find out the association between Incretin hormones, single
nucleotide polymorphisms rs1861978 dipeptidyl peptidase-4 gene polymorphism in
Type2 Diabetes Mellitus.
Subjects and Method
This is case control study for two hundred and forty Iraqi subjects. One
hundred and twenty patients who were diagnosed previously with type 2 diabetes
mellitus based on physicians diagnosis prior to their current visit to Al-Hassan
diabetic and endocrinology care center in Imam Hussein medical city/holy Kerbala
city/Iraq. Some of them were taking medications and others were not. They all have
no obvious renal and liver problems. One hundred and twenty apparently healthy
individual candidates as control group matched in age and BMI with patients, They
have neither symptoms nor signs of diabetes and they are apparently healthy.
Fasted blood samples were collected from all patients and health subjects
during the period from January to April 2018 for measuring the level of Incretin
hormones (GIP) in addition to fasting serum glucose, and lipid profile. Two mls of
these samples were collected in EDTA tubes for DNA extraction and polymerase
chain reaction.
The DNA extraction and PCR were done at the research laboratory of the
biochemistry department of the Medicine College of Kerbala University.
Results
There was an elevation in serum fasting glucose, Triglyceride, total cholesterol
and low density lipoprotein, and decrease in serum high density lipoprotein in
Diabetic patient as compared to those of the control group.
The Diabetic patient in this study showed a significant association between single
nucleotide polymorphisms (rs1861978) in dipeptidyl peptidase-4 gene (genotype
G/G) and incretin hormone (GIP).
Conclusion
The DPP4 allelic distributions and their association with incretin hormones (GIP)
were investigated. In Iraqi type 2 diabetic patients, which may have clinical
significance.
The association between SNP rs1861978 in dipeptidyl peptidase-4 (DPP-4) gene
and incretin (GIP) was significant.
Incretin Levels in Diabetic patients showed no significant association with control.
Incretin hormones glucose-Dependent Insulinotropic Polypeptide (GIP), fasting
serum glucose and HbA1c level were increase significantly in diabetic patients in
comparison with healthy subjects.