التوظيف الحيوي لبكتريا Bacillus subtilis في السيطرة على إصابة حبوب بعض المحاصيل ألاقتصاديه بالفطرين Aspergillus flavus و Aspergillus parasiticus

Vital employment of bacterium Bacillus subtilis in controlling the infection of some economical crops grains fungi Aspergillus flavus and Aspergillus parasiticus

Summary
These included study the possibility of producing Bioformula from bacteria Bacillus subtilis in order to use it and traded commercially economically by the beneficiaries, after selecting the fermenting media most efficient in the development of bacteria and the use of a calcium carbonate as a holder for the manufacture of the product vital , and when you test a number of extracts of grains ( wheat, barley , maize ) Kosat Tkhmrah for the development of Bioformula bacteria B.subtilis maize extract showed high efficiency compared to the rest Abstracts The number of bacterial cells 810×2.15 colony formation units / ml , and calcium carbonate were efficient as the carrier of the Bioformula bacteria B.subtilis as the number of bacterial cells 810×70 colony forming unit per / g and gave the proportion of 100 ml cente 50 gm material bearer highest number of bacterial cells reaching 810×88 colony forming unite / ml, and when testing the efficiency of isolates of bacteria Bacillus subtilis in the inhibition fungle growth for Aspergilus flavus and Aspergillus parasiticus isolated from grain subject of the study , which confirmed the results of polymerase chain reaction technique (PCR) contain AflR gene responsible for the production of Aflatoxins found that B.subtilis BM1 isolation is competent than the rest of the isolates in the inhibition of both fungi reached 100 % Experience storage has shown efficient preparation Bioformula in protecting the grains of wheat , barley and maize from Aspergillus flavus and Aspergillus parasiticus as decreased incidence of 100% in the treatment control (pills treatment Balaftran separately only) to the incidence ranging between ( 20-26 %) in cereals tested
( wheat , barley, maize ) and the treatment of the Bioformula addition to prevent contamination of grains of wheat and famous aflatoxin B1 while the reduced amount of this toxin in grain maize from 800 microgram/ kg grain in the treatment of control to 310-190 microgram / kg grain , respectively, treatmentes- AF + BM1 and AP + BM1 under the conditions of storage of natural and for a period of three months. proved study the physiological and histological animals tested ( rat white) and the treatment of the Bioformula plant does not possess ofany toxic effects of animals tested