العلاج الموضعي لمرض السعفة في الانسان بواسطه تركيبه جديده من الامفوتريسين بـ باستخدام الحيوان كنموذج

Topical treatment of human Dermatophytoses by new formula of Amphotericin B using of animal model

Abstract

There is actually no part of the world can be cleared from infection with dermatophytosis. The skin, hair and nail of all types of mammalian, including the human are under the risk to develop dermatophytosis. The disease is mainly caused by different species of dermatophytes within the cutaneous layer of the skin. Several topical and systemic antifungal drugs are used for treatment of dermatophytosis. Amphotericin B (AmB) is widely intravenous used for treatment of systemic fungal infection. Topical formula of AmB is still under experimental level.
In this study, topical Amphotericin B cream 1% (AmB) was prepared to use as treatment for dermatophytosis in animal and human. Rabbits were chosen to be an animal model for treatment by new preparation of AmB cream. A total of 12 healthy rabbits were infected by clinical isolate of Trichophyton mentagrophytes. Rabbits were divided into four groups with 3 rabbits in each. First group was treated twice daily with prepared AmB cream 1%, second group with twice daily clotrimazole cream 1%; third group with cream only; and forth group was left without treatment as a control. The curative time of treated animal by AmB was found shorter (4-6 days) than by clotrimazole cream (14-28 days) with significant differences (p< 0.05).
Two groups of 12 patients with dermatophytosis were involved in this study. First group of six patients was treated with prepared topical AmB 1%, while other six participants were treated with clotrimazole cream 1%. As with treated animals, the curative period of AmB was shorter (10 day) compared with clotrimazole cream (14-21 days) with significant differences (p< 0.05).
Tinea corporis was the most prevalence type of dermatophytosis (91.66%) in involved patients, followed by tinea barbae (8.33%). Microsporum canis was more frequently isolated (83.32%) from patients with tinea corporis, while T. mentagrophytes was isolated from one patient (16.66%). Males at age range 20-50 years were more infected by dermatophytosis than females (18-52 years). Clinical features of dermatophytosis ranged from mild to severe for most involved patients in this study. Occupation type of males and females was variable between students and other type of jobs. Some of involved patients were in contact with animals and birds.