الفعالية الضد بكتيرية لمستخلصات بعض النباتات الطبية ضد البكتريا المرضية المتعددة المقاومة للأدوية والمكونة للغشاء الحيوي والمعزولة سريرياً

Antibacterial activity of extracts of some medicinal plants against multi-drug resistant (biofilm forming)pathogenic bacteria clinically isolates

120 bacterial isolates were obtained from 128 swabs of patients with tonsillitis in the holy city of Karbala, and after conducting tests by biochemical diagnostic methods, it was found that these isolates include Staphylococcus Spp, Streptococcus Spp, Klebsiella pneumoniae, E. coli, Enterobacter Spp, Pseudomonas Spp, and the highest numbers were Staphylococcus aureus numbered 19 isolates and 18 isolates of Klebsiella pneumoniae. When these isolates were subjected to an antibiotic sensitivity test by measuring the diameter of inhibition around the discs, all of them were multi-drug resistant (MDR).
It appeared that there are (6) isolates of S. aureus and (7) isolates of K. pneumoniae that have a strong ability to build biofilm after examining them by titer plate – Micro plate (MTP) test.
After subjecting the selected isolates of S. aureus and k. pneumoniae assay using the VITEK-2 compact system, the diagnostic results appeared to be identical to the initial diagnosis and to the results of the antibiotic susceptibility test.
The inhibitory effects of the hot aqueous extract of Green Tea, Carnation, Liquorice, and Cinnamomum verum were investigated at concentrations (10, 20, 40, 60, 80, and 100) mg/ml. aureus compared with the rest of the remaining plant extracts, and the rest of the extracts did not show any activity against K.pnuemoniae bacteria isolates.
The Minimum Inhibitory Concentration (MIC) value for green tea extract was determined at a concentration of 8 mg/ml against S. aureus and 36 mg/ml against K.pneumoniae.
The cytotoxicity of green tea extract was also studied, as the percentage of toxicity was 8%. It was found that the results were good and there was no hemolytic activity against human red blood cells, as Green Tea leaves consumed naturally and in doses ranging from one or two cups per day are safe and not It results in no toxicity or harmful effects, due to the ability of the phenolic compounds present in Green Tea to protect red blood cells from decomposition. The cytotoxicity of green tea extract was also studied and the results were good, as green tea leaves consumed naturally and in doses ranging from one or two cups per day are safe and do not result in any toxicity or harmful effects.