الفعالية المضادة للصمغ العربي في نمو البكتريا المرضية المعزولة من المرضى المصابين بألتهابات المسالك البولية

Antibacterial Activity of Arabic Gum Against Some Pathogenic Bacteria Isolated from UTI Patients

abstract

The urinary tract infection is a widespread bacterial infection; it is one of the major problems suffered by humans, and responsible for many health problems. So many studies have sought to find the appropriate solutions to reduce the spread of pathogen in society.
During the period extending between May 2022 to July 2022, urine samples were obtained from 53 suspected UTI patients and 35 healthy controls, their ages were ranged from 3 to 69 years old. Collection of samples were carried out in two places (Al-Hussain Teaching Hospital and private clinic).
The bacterial growth was identified by using Vitek system. The Human Cathilcidin (LL-37) and Human lactoferrin were measured by using the ELISA system, The results showed that the percentage of infection in females was higher than in males where the infection rate in females was 50% ,but in males was 49% .In addition, the highest rate of infection was in the 20-29 age group while the lowest infection rate was in the 60-69 age group. Out of 88 urine samples, the results also showed that 2 (3.8%) had no bacterial growth and 51 (96.2%) had considerable bacterial growth.
The results of bacterial isolates were 16/51 (30.2%) Escherichia coli, 14/51 (26.4%) Staphylococcus aureus, 10/51 (18.9%) Klebsiella pneumoniae, 6/50 (11.3%) Enterococcus faecalis, 5/51(9.4%) pseudomonas aeruginosa .
The mean of urinary LL-37 levels showed significant differences (P≤0.05) between patients (1.96) and control (1.72). Also there was a significant difference (P< 0.01) between the levels of LTF and LL-37 with bacterial isolate. Where higher concentration was recorded in patients with E.coli (2.8), followed by klebsiella pneumonia (2.18), Enterococcus faecalis (1.77), Staphylococcus aureus (1.45) ,and Pseudomonas aeruginosa (0.88).
In Vitro effect of aqueous Arabic Gum solution against S.aureus and E.coli was estimated by well diffusion method at concentrations ranged from 100 mg/ml to 500 mg/ml ; the results revealed that the aqueous Arabic Gum solution had the antibacterial activity against the studied E. coli and S. aureus bacteria. The mean of inhibition zone diameter of E.coli isolates increased with concentration of aqueous Arabic gum solution increased to reach to 11.7 mm in the high concentration of aqueous solution 500 mg/ ml , but in low concentration 100 mg/ ml was there was no effect. While inhibition zone diameter against S.aureus bacteria reach to 10 mm in the high concentration of the aqueous solution 500 mg/ ml, but in low concentration 100 mg/ ml there was no inhibition zone. The aqueous solution of Boswellia Serrata was also used, but it did not give any antibacterial activity on the bacterial isolates on the bacterial isolates.