الكشف الكیمیائي النسیجي المناعي لعامل نخر الورم الفا وانترلوكین ٤ مع الكشف الجزیئي لبكتریا الملویه البوابیه في التھاب المراره الحصوي المزمن

Immunohistochemical detection of TNF-α and IL-4 with molecular detection of Helicobacter pylori in chronic calculus cholecystitis

Abstract

cholelithiasis is one of the most prevalent digestive disorders which is
consider as causative agent to induce gallbladder inflammation (cholecystitis). TNF- α as Pro-inflammatory cytokines, play an important role in initiation of
inflammation. However, anti- inflammatory IL-4, is a pleotropic cytokine
stimulation of activated B-cell and T-cell proliferation. On other hand it is well
established that Helicobacter pylori has pathogenic role which may infected over
50% of people around world. In addition, it is known to induce gastrointestinal
disorders. So, this study has undertaken to reveal the relationship between H. pylori
and cholecystitis explaining development of gallstones formation.
Current study was performed on 95 patients which were clients in surgical
clinic in Al-Husain General Hospital at Al Nasiriyah province. Duration of study
was from November to December 2018. all included patients were subjected to
interview for filling questionnaire. gallbladder tissues have been divided in two
parts, first pieces were preserved in 10% formalin for embedding-paraffin section
procedure to conduct routine stain and immunohistochemical (IHC) protocol. Two
types of cytokines were included as antibodies in IHC protocol (TNF-α and IL4).
Second pieces of gallbladder tissue have been immediately collected and kept
by frozen in -20ºc for real time pcr protocol. Real-Time PCR technique was
performed for detection of Helicobacter pylori based on amplification UreC gene in
gall bladder samples after cholecystectomy.
Results showing Out of the 95 patient’s immunoexpression of TNF-α was
high while IL-4 had low expression in chronic calculus cholecystitis. Also, it has
been found that there was no significant correlation between TNF-α and IL-4 among
CCC and CAC patients and there is (positive correlation) between grading and TNF- I
α. as well as, there is positive correlation between grading and IL-4. In real time pcr
find (32.6%) were positive for H. pylori UreC gene.
Also, there was no significant association in TNF-α expression among
positive and negative H. pylori groups.
In association between IL-4 and H. pylori infection there was no
significant association was found between groups.
Taken together, these results concluded that increasing of localization of TNF-
α and decreasing of localization IL-4 in gallbladder tissues have role on developing
pathogenesis of cholelithiasis. Current study confirmed that The presence of H.
pylori in gallbladder mucosa might indicate a big risk for cholelithiasis