المعالجة الأحيائية لسم الزيرالينون المفرز من قبل الفطر Fusarium verticillioides الملوث لحبوب الذرة الصفراء بإستخدام البكتريا Bacillus subtilis و Lactobacillus plantarum

Preparation and Characterization of Nanohybrid Antibiotic from Levofloxacin and Evaluation its Inhibitory Efficiency against Bacteria Isolated from Diabetic Foot Ulcer

         This study aimed to isolate the bacteria thats found in dairy and evaluate their activity in detoxification of zearalenone toxin(In vitro) and then select the the most efficenceone of bacteria in detoxification and reducing zearalenone toxicity (In vivo)

         Isolation and diagnosiste identificalt tests showed Aspergillus spp. and Fusarium spp. appearance were 96% and  32% respectively.

         Also results of Fusarium spp.  have ability to produce test on the production of zearalenone toxin by Thin layer chromatography(TLC) appeared at rate by 72.22%.

         diagnosis of isolates produced by zearalenone was using polymerase chain reaction (PCR). The results of the nucleotide sequence analysis of the double nucleic acid package showed that the isolates produced were due to Fusarium verticillioides.On the other side three bacterial types of dairy were isolated and diagnosed were Lactobacillus plantarum,Lactobacillus rhamnosus and Streptococcus thermophilus.As well as study of activity  of bacteria Bacillus subtilis in protection of yellow maize seeds from F.verticillioides under natural storage percentage of from 100% infecton to 48.88%for seeds treated with fungus after storage period for three months   .          L.plantarumshowed activity in reducing the toxicity of zearalenone externally(In vitro)when shine spotsof toxin zearalenon disappearedthats treatedpreviously with bacteriaby test of plateThin layer chromatography(TLC) and exposed to UV at a 360nm wavelength, then it was strengthned  this result by in vivo bio assay of albino male Rats and the transactionof zearalenone pre-treated with bacteriaL.plantarum and dairy.The physiological blood parameters were completely healthy but showed clear variances in the physiological blood parameters in animals treated with zearalenone only. The count of red blood cell in the treatment  bacteria and toxin,dairy and toxin 6.66×10⁶ cell/mm³and 6.83×10⁶ cell/mm³ respectively. While its numbers decreased in the treatment of toxin only,reached 4.81×10⁶ cell/mm³The number of lymphocytes in the treatment of bacteria and toxin, dairy and toxin 10.13×10³ cell/mm³ and 10.17×10³ cell/mm³ respectively and their numbers in the toxin treatment decreased to 7.74×10³ cell/mm³the most recent zearalenone toxin reduced the amount of hemoglobin(Hb) in the blood of animals treated with the toxin zearalenone only at 8.5 g/100ml. There were important role ofL.plantarum and dairy in maintaining normal hemoglobin levels in animals,which treated individual then followed by the toxin zearalenone ,amount at 14.06 g/100ml and 13.47 g/100ml respectively.The results explained the ability of L. plantarum bacteria to protect the tissues of treated animals, which were Oraldosage with the toxin zearalenone after that results of the histological examination of tissue sections for the organs hncludedheart, intestines, liver, kidney and spleen safety of any disease, while showed clear changes in all of these tissues Members of animals treated with the toxin zearalenone only.