تأثير المركبات النانوية المحملة بالميثوتريكسات ومستخلص القرفة في بعض المعايير المناعيـة في ذكور الجرذان المستحث بها التهاب المفاصل الرثياني وفي نمو بكتريا Pseudomonas aeruginosa

Effect of nanocomposites loaded with methotrexate and cinnamon extract on some immunological parameters in male rats induced with rheumatoid arthritis and on the growth of Pseudomonas aeruginosa bacteria

Abstract
The present study aimed to evaluate the anti-arthritic effects of the Cinnamomum zeylanicum nanocomposite. Rheumatoid Arthritis (RA) was induced by injecting Complete Freund’s Adjuvant (CFA) (0.1 ml) into the right paw of 42 male albino rats. Stable silver nanoparticles with Cz significantly reduced the edema of the chelate and changes in weight as the nanocomposites from cinnamon extract and silver nitrate Ag(NO)3 were prepared and diagnosed. The loading process was successful, and the Atomic Force Microscope (AFM) image demonstrated the appearance of changes in the surface of the nanocomposites loaded with the treatment, and the radiographic evaluation showed the severity of the joint deformation clearly according to the extent of osteoporosis and joint spaces.
The immunological results indicated a significant increase (P<0.05) in the levels of cytokines IL-1β, IL-8, IL-6 and blood picture parameters both in the positive control group G2 compared to the negative control group G1 and there was a significant decrease in the level of concentrations. These cytokines were in the groups after being treated with cinnamon extract and cinnamon nanocompound before and after treatment loading, and when comparing the groups treated with cinnamon nano compound loaded with the treatment under study for half the period with groups treated with free MTX treatment for the entire treatment period, a significant decrease was found in the concentrations of these cytokines that led to a shortening of time And the amount of treatment is almost halved, evidence of an improvement in treatment efficiency by 50%.
The inhibitory effect on the growth of Pseudomonas aeruginosa was studied by the cinnamon nanocomposite in its free and loaded form, and the results were compared with the inhibitory ability of the antibiotic Erythromicin. A significant increase in the diameter of the inhibition ring was found in the groups treated with the Cinnamon nano compound loaded with the antibody (22.75) mm Cinnamon nano has an inhibitory effect on these bacteria and this effect led to an improvement in the efficiency of the antibiotic Erythromicin.