تأثير المركب النانوي كاربونات الكالسيوم @العلك العربي على امصال مرضى السكر النوع الثاني والتهاب المفاصل العظمي

Effectiveness of Calcium Carbonate@ Arabian Gum Nanoparticles on Sera of Diabetes mellitus Type II with Osteoarthritis Patients

Abstract

Diabetes mellitus type2 and Osteoarthritis are the most common diseases particularly in Iraq and in the world. The present work was carried out in Rheumatology department in AL-IMAM Al- Hassan Hospital in holy Karbala city at 90 women (50 women with osteoarthritis and diabetes mellitus type2 as patients group and 40 women apparently healthy as a control group (all of them aged from 40-70). The aim of this study is to study the differences of blood glucose, hemoglobin A1C, high sensitivity C-reactive protein, serum calcium, vitamin D3, parathyroid hormone, serum albumin and alkaline phosphatase in patients of T2DM with OA compared to control group in Iraqi women by measuring those parameters and study the effect of the Calcium Carbonate@Arabian Gum Nanoparticles on sera of the patients group. The work included two parts. The first part was measuring the parameters that diagnose the two diseases, which were fasting blood sugar and hemoglobin A1C for diabetes mellitus while for osteoarthritis had been measured serum calcium, vitamin D3, parathyroid hormone, high sensitivity C-reactive protein, serum albumin and alkaline phosphatase. The second part included preparation Calcium Carbonate@Arabian gum Nanoparticles by Co-precipitation method and characterized by scanning electron microscopy, transmission electron microscopic, X-ray powder diffraction and investigated by UV−Vis-NIR spectra. Calcium Carbonate@Arabian gum Nanoparticles were added to1ml pool of sera of 15 patients in different concentrations and the results showed that there was a significant difference between patients group and controls group in blood glucose, hemoglobin A1c alkaline phosphatase, parathyroid hormone and high sensitivity C-reactive protein (p<0.05). There were significant differences in Alb and calcium (p<0.01). Albumin negatively significant with Parathyroid hormone (P<0.05). Significant differences in high sensitivity C-reactive protein and
phosphorus(P<0.05). The results after adding CaCO3@AGNPs and CaCO3NPs showed a decrease in the levels of blood glucose, alkaline phosphatase, albumin, parathyroid hormone, and phosphorus in the blood. It was also observed that the level of calcium increased. It could be concluded that there is a high association between diabetes mellitus type tow and osteoarthritis disease and the Synthesized Calcium Carbonate@Arabian Gum NPs is very effective in reducing glucose levels. Calcium Carbonate NPs can provide the calcium easier than Carbonate@Arabian Gum NPs.