تأثير المستخلص الكحولي لأوراق نبات عين البزون في بعض المعايير الوظيفية والنسجية لذكور الجرذ الأبيض المعاملة بمادة الألوكسان تجريبيا

Effect of ethanol extract of leave Vinca rosea plant in some functional and histological on males white rats treated with alloxan experimentally

   This study aimed to determine the effect of  ethanol extract of the leaves Vinca rosea plant on some functional and histological standards effects in male white rat infected diabetic development with alloxan.  .

     The study was conducted in the Animal house of biology Department in the College of Education Pure Science – University of Karbala for the period from Decemper 2013 and until the month of August 2014, has been the use of number 75 male rats and randomly divided into five groups comprising (15 animals per group) the first group G1 is control group and it was dosaged daily with a solution of Physiological normal salin for two months and promised to set control is negative,the second group G2 was induced diabetes by injected its by alloxan with 150 mg /kg of body weight under intraperitoneal and promised to set control is positive, while the third ,fourth and fifth groups have been induced diabetes before of the dosage by ingected with alloxan and dosage orally after month from induced diabetes with ethanol extract of leaves Vinca rosea by 150,200 and 250 mg/kg of body weight per aday for amonth, respectively.

       Blood samples were collected from all groups before the induced diabetes, a month after the induced diabetes , and after a month of dosing ethanol extract of Vinca rosea for the study of the following criteria: level measurement of Glucose , measure the level of the hormone insulin, blood Hemoglobin(Hb), measure the number of Red Blood cells(RBC), measuring the total number of white Blood cells (WBC) cells, measuring the Packed Cell Volum(PCV) ,measuring the concentration of total cholesterol in the blood (TC) , triglyceride (TG) Triglycerides and the concentration of high-density lipoproteins (HDL-C) , low density lipoproteins (LDL-C) , very low density lipoproteins(VLDL-C) ,the level of liver enzymes Aspartate transaminase(AST), Alanine transaminase(ALT), Alkaline phosphatase (ALP),Ceratine Kinase –MB, measuring level creatinine and urea , as well as taking the histological sections of the liver , kidney, pancreas and heart to purpose of study histologic changes them after measuring the weights of these organs except pancreas the results showed :

1-The induced diabetes led to asignificant increase p<0.05 in the level of Glucose, significant decrease p<0.05 in the concentration insulin hormone compared with the control group.The significant decrease p<0.05 in level of Glucose ,and high moral p<0.05 in concentration of insulin hormone groups in treatment with ethanol extract of Vinca rosea compared to the control group .

2- The induced diabetes led to a significant decrease P<0.05 level Hb,and number of R.B.C and the level ​PCV and significant increase P<0.05 level in W.B.C compared to control .And get high moral P<0.05 in level Hb ,number R.B.C and the level PCV and significant decrease P<0.05 in number of W.B.C groups in treatment with ethanol of Vinca rosea compared to the control group.

3-The induced diabetes led to a significant increas P<0.05 in the concentration of TC, TG, LDL, VLDL and effectiveness of the enzymes and liver function AST,ALT,ALP, CK-MB and significant decrease P<0.05 in the concentration of HDL compared with the control and get significant decrease P<0.05 in the level TC,TG, LDL, VLDL and effectiveness enzymes and liver function AST, ALT, ALP, CK-MB and high moral P<0.05 in the concentration of HDL groups treatment with ethanol extract of leaves Vinca rosea compared the control group.

4- The induced diabetes led to a significant increase P<0.05 in the level of creatinine,and urea compared with the control group . The significant decrease P<0.05 in the level of creatinine,and urea groups in treatment with ethanol extract of leaves Vinca rosea compared to the control group .
5-The induced diabetes led to significant increase P<0.05 in the weights of liver, kidney and heart compared with the control group .high moral P<0.05 in liver weight and kidney and heart at the treatment with ethanol extract of leaves Vinca rosea compared to the control group .

6- The induced diabetes led to additional changes in the liver, kidney and pancreas of infected animals compared with the control group, as shown in the liver multiple regions of the hepatic lobules congestion bloody obvious in the region pylori with infiltration of inflammatory cells to post an average power vaculation simple in hepatic cells with the expansion and irregular sinusiods ,A treatment with ethanol extract of leaves Vinca rosea showed protective effect of the liver and explained the installation of histological closer to normal except for the presence of infiltration of cells and simple congestant .when led the induced diabetes to additional changes in the kidney as showed its by the bloody congestion and atrophy of the size of the glomerulus and degeneration in the tubules and the presence of protein in the renal tubules and the expansion of Bowman’s capsule. A treatment with ethanol of leaves Vinca rosea extract showed protective effect of the kidney by the reducing of degenerative changes and glomerulus-size close to normal.while led the induced diabetes to additional changes in the pancreas find decreased size and atrophy of lankerhanz islets and degeneration of cells and vaculation interacinous.Atreatment groups with ethanol extract of leaves Vinca rosea showed change lankerhanz Islands lankerhanz close to the natural shape of the cells in size secreted cells with bloody congestion in the glandular part with irregular shaped island . the results of the study showed no significant changes in heart tissue of infected animals and the treatment with ethanol extract of Vinca rosea  plant .