تأثير المستخلص المائي الحار لنبات الشيح على بعض المعايير الوظيفية والنسجية لبعض اعضاء ذكور الجرذ المستحث بها داء السكر

Effect of Hot Aqueous Extract of Artemisia herba alba Plant on Some Functional and Histological Parameters In Some Organs in the males Rats with Induced Diabetes

   This study aimed to determine the effect of aqueous extract of Artemisia herba alba plant on some physiological , and histological standards effects in male white rate infected diabetic development with  alloxan .

          The study was conducted in the Animal house of biology Department in the College of Education Pure Science – University of Karbala for the period from December 2013 until the month of August 2014 , has been the use of male rat local the 75 male and randomly divided into five aggregates comprising  ( 15 animals per group ) the first group G1 is control group and it was dosaged daily with a solution of Physiology normal salin for two months and promised to set control is negative, the second group G2 was developed diabetes by injected its by alloxan with 150 mg / kg of body weight under intraperitoneal and promised to set control is positive , while the third , fourth and fifh groups have been induced diabetes before of the dosage by ingected with alloxan and dosage orally after month from induced diabetes with aqueous extract of Artemisia herba alba by 500 , 1000 and 1500 mg/kg of body weight per a day for a month , respectively .Blood samples were collected from all groups before induced diabetes, a month after induced diabetes , and after a month of dosing aqueous extract ofArtemisia herba alba for the study of the following parameters : level measurement the number of Red blood corpscules count (R.B.C), Blood Hemoglobin (Hb), Packed Cell Volume(%PCV)   measuring the total number of White blood cells count (W.B.C) ,and measuring the concentration of Glucose in the blood and hormone insulin and total cholesterol in the blood (TC) , triglyceride (TG) and the concentration of high-density lipoproteins (HDL-C) , low density lipoproteins (LDL-C) , very low density lipoproteins (VLDL-C) , the level of liver enzymes Aspartate transaminase (AST), Alanine transaminase (ALT) , Alkaline phosphatase (ALP),Ceratine Kinase –MB, and level of creatinine and urea , as well as taking the histological sections of the liver , kidney, pancreas and heart to purpose of study histologic changes them , after measuring the weights of these organs, except pancreas the results showed :

1 -The Results of the study showed that the development of diabetes in male rats led to a significant decrease( P <0.05) the number of R.B.C and  level Hb, and, and (%PCV)significant increase (P <0.05) level  in W.B.C compared to the intact control group , And get high moral (P <0.05) in the number of R.B.C and Hb , high non significant (P> 0.05) in the level of %PCV and significant decrease  P <0.05 in the number of W.B.C aggregates in treatment with aqueous extract of Artemisia herba alba plant compared to the infected control group .

2 – The results of the showed, the development of diabetes in male rats led to a significant increase (P <0.05) in the concentration glucose, TC, TG, LDL, VLDL and effectiveness of the enzymes and liver function AST, ALT, ALP and urea and creatinine  and significant inecrease (P >0.05) of CK-MB and significant decrease P <0.05 in the concentration of HDL and the concentration of insulin hormone compared with the intact control and get significant decrease (P <0.05) in the concentration of glucose TC, TG, LDL, VLDL and effectiveness enzymes liver function AST, ALT, ALP and urea and creatinine with significant decrease (P >0.05) of CK-MB ,high moral (P <0.05) in the concentration of the insulin hormone and high significant (P< 0.05) HDL aggregates in treatment with aqueous extract of  Artemisia herba alba compared to the infected control group

3- Results of the study showed that the development of diabetes in male rate led to a rise  significant P<0.05 in the liver ,kidney and heart weights compared with the intact control group , high moral P <0.05 in the weight of the organs at the treatment with aqueous extract of Artemisia herba alba  , compared to the infected control group.

4-The results of the study showed that the development of diabetes in male rat White led to obtain changes in the liver of infected animals compared with the healthy control group, As observed in multiple regions of the hepatic lobules congestion bloody obvious in the region pylori with infiltration of inflammatory cells vaculation simple in hepatic cells with expansion and irregular Sinusiods compared with the negative control group, Showed the group treatment with aqueous extract of Artemisia herba alba protective effect of the liver with  activation  ofKupffers cell

5– The results  of study showed that the development of diabetes in male rat White led to obtain changes in the Kidney of infected animals compared with the control group, as shown by the atrophy of the size of the glomerulus with clear expansion in the low bed Bowman , congestion of blood vessels with degeneration in the area tubules was observed also the presence of the protein deposited materials in the renal tubules. aqueous extract of Artemisia herba alba Protective effect of the Kidney  through the reduction of degenerative changes and glomerulus-size close to normal. As also showed the group treatment aqueous extract of Artemisia herba alba dose of 1000,1500 mg / kg changes histology includ atrophy of the size of the glomerulus with the expansion of the Bowman’s capsule and congestion bloody simple and vaculation cell in the tubule .

6- The results of the study, showed the development of diabetes in male rat led to additional changes in of lankerhanz islands in infected animals compared with the intact control group , as demonstrated for changes in the tissues of the pancreas and decreased size of the lankerhanz islets to and degeneration of cells . While treatment groups with aqueous extract of Artemisia herba alba  showed change lankerhanz Islands langerhanz close to the natural shape of the cells in size,secreted cells. While observed after treatment 1500 mg / kg of the plant extract atrophy in the size of Islands Lnkerhanz with irregular shaped island.

7- The results of the study , showed significant changes in heart tissue of infected animals and the treatment with with aqueous extract of Artemisia herba alba  plant .