تأثير المستخلص المائي لبذور الكزبرة على بعض معايير الدم الكيموحيوية والنسجية لبعض الأعضاء في ذكور الارانب المستحدث بها داء السكري

Effect Of Aqueous Extract of Coriandrum sativum Seeds On Some Biochemical , Blood and Histological parameters In Some Organs in the males Rabbits Induced with Diabetes

This study aimed to determine the effect of aqueous extract of coriander seeds on some biochemical , blood and histological parameters in some organs in the males local rabbits induced with diabetes .

The study was conducted in the Animal house of biology Department in the College of Education for Pure Science – University of Karbala for the period from May 2013 until October 2013 , has been used male rabbits local the 25 male range average weights between 1500-1750 gm and randomly divided into five groups comprising (5 animals per group) the first group G1 is control group and it was dosaged daily with a solution of Physiology normal salin for two months and promised to set control is negative, the second group G2 was induced diabetes by injected its by alloxan with 150 mg / kg of body weight under intraperitoneal and promised to set control is positive , while the third , fourth and fifh groups have been induced diabetes before of the dosage by ingected with alloxan and dosage orally after month from induced diabetes with aqueous extract of coriander seeds by 50 , 100 and 150 mg/kg of body weight per a day for a month , respectively .

Blood samples were collected from all groups before induced diabetes, a month after induced diabetes , and after a month of dosing aqueous extract of coriander seeds for the study of the following parameters : level measurement Blood Hemoglobin (Hb), measure the number of Red blood corpscules count (R.B.C), measuring the total number of White blood cells count (W.B.C) , measuring the level of Glucose, and the level of Total cholesterol in the blood serum (TC) , Triglycerides (TG) and the level of High-density lipoproteins (HDL-C) , Low density lipoproteins (LDL-C) , Very low density lipoproteins (VLDL-C) , the level of liver enzymes Aspartate transaminase (AST), Alanine transaminase (ALT) , Alkaline phosphatase (ALP), measuring level of Creatinine and Urea in addition to measure the level of effort oxidative Malondaialdehyde (MDA) and measure the level of the Hormone insulin , as well as taking the histological sections of the liver , kidney and pancreas to purpose of study histological changes them , after measuring the weights of the liver , kidney, the results showed :

1- The induced diabetes led to a significant decrease (P<0.05) in the level of Hb, and the number of R.B.C, and hihg non significant (P>0.05) in the number of W.B.C compared to the control group , And get high moral (P<0.05) in the level of Hb and in the number of R.B.C, and non significant decrease (P>0.05) in the number of W.B.C groups in treatment with aqueous extract of coriander seeds , compared to the infected control group .

2- The induced diabetes led to a significant increase (P<0.05) in the level of glucose , TC, TG, LDL, VLDL and effectiveness of the enzymes and liver function AST, ALT, ALP and significant decrease (P<0.05) in the level of HDL and the level of insulin hormone compared with the control group and get significant decrease (P<0.05) in the level of glucose , TC, TG, LDL, VLDL and effectiveness enzymes liver function AST, ALT, ALP and lower non- significant (P>0.05) at a dose 50 mg / kg of body weight in ALT and high moral (P<0.05) in the level of the insulin hormone and high non significant (P>0.05) HDL in the groups in treatment with aqueous extract of coriander compared to the infected control group .

3- The induced diabetes led to a significant increase (P<0.05) in the level of creatinine , urea and MDA compared with control group . The significant decrease (P<0.05) in the level of urea and non- significant (P>0.05) in the level of creatinine and MDA in the groups treatment with aqueous extract of coriander seeds compared to the infected control group .

4- The induced diabetes led to a rise in non- significant (P>0.05) in the liver and kidney weights compared with the control group , With the lack of moral differences between the five groups of liver and kidney weights at the treatment with aqueous extract of coriander seeds , compared to the infected control group.

5- The induced diabetes led to additional changes in the tissues liver , kidny and pancreas of infected animals compared with the control group , as showed in the liver bloody congestion and bile stagnation and cytoplasm vacuolation and greasy collect and necrosis of liver cells and not the presence of sinusiods and ngld nuclei . A treatment with aqueous extract of coriander showed  protective the liver from damage effects and explained the installation of histological closer to normal except for the presence of infiltration of inflammatory cells and a simple congestion . when led the induced diabetes to additional changes in the kidney , as showed its by the bloody congestion and glomerulus inflammation , degeneration of the renal tubules , infiltration of inflammatory cells , the presence of protein in the renal tubules , the expansion of Bowman’s capsule and increase cellular glomerulars . At a treatment with aqueous extract of coriander showed protective the kidny from damage effects by reducing degenerative changes and glomerulus -diameter close to normal . while led the induced diabetes to additional changes in the tissues of the pancreas as low numbers of beta cells and decreased diameter of the langerhanz islets and degeneration of cells . A treatment groups with aqueous extract of coriander showed change langerhanz Islands langerhanz close to the natural shape of the cells in the diameter , secreted cells and alveoli  pancreas