تأثير جرع مختلفة من الكورتزون على بعض المعايير الفسلجية والنسجية في الجرذان البيض

Effect of different cortisone doses on some physiological and histological parameters in the albino rat

Abstract

The study was involved the use of albino rats (males , females) at ( 64) mice were divided into two groups and each group was included (32) rat (16 males and 16 females) and these totals recent divided into four groups were included four rats for each group, The last three groups were injected by Intraperitoneal drug of hydrocortisone with concentrations double (1.5,3,4.5 ) mg / kg for (30,60) day and the fourth group was injected by normal Saline 0.9% as control in the same volumes that used, The study was aimed to indicate the effect of concentrations and duration of injection in some physiological and histological parameters and effects disease caused in albino rats the study included the following:
1- Statement effect of different concentrations of hydrocortisone in the total count of red blood cells , the total count of white blood cells , and Packed cell volume , and concentration of hemoglobin in the blood , the Mean corpuscular volume. Found from the study of a rise significantly (p <0.05) in the total count of red blood cell (RBC), and a significant decrease (p <0.05) in the total count of white blood cells (WBC) hemoglobin (Hb ) and Packed cell volume (PCV) and the Mean corpuscular volume (MCV) when compared to all results the control group.
2- Measurement biochemical parameters in serum and fibrinogen in plasma to show the effect concentrations of injection by drug of hydrocortisone, the results were showed a rise significantly (P<0.05) in the effectiveness of enzyme of liver (AST, ALT) as well as a significant increase (P<0.05) in the concentration of protein albumin and fibrinogen, and the lack of significant (P <0.05) changes in urea when comparing the treatment groups with the control group.
3 – To identify the effects of histopathological in the tissues of the spleen , liver and bone , where we note a degeneration in lymphocyte and an increase in the preparation of the phagocytic cells and lack in the number of white pulp in the spleen and small size , while the liver we notice a necrosis of liver cells and infiltration of inflammatory cells and the appearance of fatty change with in the hepatocyte, in the bone tissue note for the lack of bone in the thickness of the trabeculae and increase the distance between these trabeculae.