تأثير داء السكري المستحدث على بعض المعايير الوظيفية والنسجية في حوامل إناث ومواليد الجرذ الأبيض

effect diabetes Induced on some functional and histological standards in pregnant females and borns in the White Rat

The aim of this study was to investigate the effect of diabetes Induced on some functional and histological standards in pregnant femals and borns white rat.

      The study was conducted in the house Animal of the Department of biology in the College of Education, Science Pure – University of Karbala for the period from December 2012 until June 2013, has been used female rat white the 72 female and were randomly divided into three groups comprising (24 animals per group) Group T1 a group of control divided into 8 females were drawing blood from before pregnancy and 8 females were injected on the sixth day of pregnancy with a solution of salt Physiological intraperitoneal was drawing blood from day 18 of pregnancy and 8 females remained delivery, the second group T2 were induced diabetes by one month before pregnancy were injected with Alloxan and a concentration of 150 mg / kg of body weight intraperitoneal and divided into 8 females were drawing blood from before pregnancy and 8 females were drawing blood from day 18 of pregnancy and 8 females remained delivery, and the third group T3 divided into 8 females were drawing blood from before pregnancy and 8 females were induced diabetes were injected on the sixth day of pregnancy Alloxan a concentration of 150 mg / kg of body weight intraperitoneal blood was withdrawn them day 18 of pregnancy and 8 females remained delivery.

      Blood samples Collected in the period before conception and day 18 of the study of the following criteria: measuring the concentration of Glucose, the concentration of total cholesterol in the blood (TC) and Triglyceride (TG) High density lipoproteins (HDL-C) Low density lipoproteins (LDL-C) and very low density lipoproteins (VLDL-C) and effectiveness of the enzymes liver function Aspartate transaminase (AST), Alanine transaminase (ALT) and Alkaline phosphatase (ALP) Submitted to measure the concentration of estrogen and progesterone, as well as taking the Histological sections of the pancreas and the liver for the purpose of examining the histological changes, the results of the current study showed:

1 – The results of the study showed that induced diabetes in pregnant female rat led to a significant decrease of( P <0.05) in the average weights of pregnant female rats compared to the control group.

2 – The results of the study indicated that induced diabetes in rats before pregnancy T2 and during pregnancy T3 led to a significant high (P <0.05) in average birth weights compared to the control group T1.

3 – Results of the study showed that the induced diabetes in rats before pregnancy T2 and during pregnancy T3 led to a significant decrease of           (P <0.05) in the average number of births compared to the control group T1.

4 – The results showed that the induced diabetes in rats before pregnancy T2, during pregnancy T3 led to a significant high (P <0.05) in the concentration of Glucose, TC, TG, LDL, VLDL and effectiveness of the enzymes liver function AST, ALT, ALP and a significant decrease P <0.05 in the concentration of HDL and estrogen and progesterone in serum compared with control T1.

5 – The results of the study, induced of diabetes in female rat pregnant led to obtain changes in the islets of Langerhans in the pancreas births compared with births of female control group, as shown for changes in the tissues of the pancreas as the abundance of alpha- cells and low numbers of beta-cells and the appearance of gaps with in islets of Langerhans.

6- The results of the study showed that the development of diabetes in pregnant female rats led to changes in the liver of births compared with female born of the control group, as shown by the congestion of blood vessels in the central vein and hepatic necrosis of liver cells and not the presence of Sinusoids and ngld nuclei.

     Inferred from the current study that diabetes Induced effect on some of the functional and histological standards in pregnant female and borns white rat.