تأثير مركبات RH-SiO2-Ag+ النانوية من مخلفات الرز ضد بكتريا Streptococcus mutans المعزولة من مرضى تسوس الاسنان

The effectiveness of RH-SiO2-Ag+ nanoparticles derived from RiseHuske plant in inhibiting Isolated Streptococcus mutans from caries patients

This study included isolating and diagnosing the most important bacteria that cause dental caries, as the isolated bacteria were diagnosed by conventional methods. The aforementioned prepared nanocomposites against bacteria isolated in this study showed the following; From a total of 100 samples taken from the mouth of patients suffering from dental caries in the health center in Al-Abba’s neighborhood in Karbala from the period 2-3/2022 to 3/6/2022, 45 isolates of streptococcus mutans. the nanocomposites extracted from rice husks were used as an antibacterial and yeast, and the nanocomposites were identified by a number of physical techniques, as the results of the FT-IR spectroscopy showed a shift in the frequencies of some chemical groups towards high and low frequencies. X-ray spectroscopy (XRD) also revealed the emergence of new levels of diffraction in the spectrum of nanocomposites, and it was clear from the results of the examination by atomic force microscopy (AFM) that the average dimensions of the diameters of the particles of nanocomposites were different, while the examination using scanning electron microscopy (SEM) revealed the appearance of irregular spherical and semi-spherical shapes. The nanocomposites prepared from rice husks were used as antibacterial, and the inhibitory activity of the nanocomposites was studied in this study against isolates of streptococcus mutans. isolated in this study as well, the results revealed the detection of the inhibitory activity of the nanocomposites against the isolates of S. mutans bacteria. This was done using each of the nanocomposites (RH-SiO2, RH-SiO2-Ag+, RH-SiO2-Ag0G, and RH-SiO2-Ag0s) prepared from rice husks at multiplying concentrations of 8 to 1024 mg/ml. The isolates of S. mutans bacteria were less sensitive to these nanocomposites, complying with the lowest diameter of inhibition at a concentration of 8 and 16 µg/ml and more resistant to nanocomposites prepared at a concentration of 1024 µg/ml. This was done using each of the nanocomposites (RH-SiO2, RH-SiO2-Ag+, RH-SiO2-Ag0G and RH-SiO2-Ag0S) prepared from rice husks, As the results showed, the toxicity analysis of the prepared nanocomposites revealed that they did not contain a prominent hemolytic component, where the RH-SiO2 nanocomposite gave a percentage lower than the minimum value of hemolytic activity. The hemolytic activity of the crude poison reached 0.4%, and thus it is non-toxic. As for the nanocomposite RH-SiO2Ag+, it also gave a result of 1.6% less than the minimum level of hemolytic activity, and thus it is considered non-toxic as for the nanocomposite RH-SiO2Ag0G, it is also non-toxic, with a toxicity value of 0.8. The RH-SiO2Ag0s nanocomposite is also non-toxic, as the hemolysis test value was 1.064%, which is less than the minimum hemolytic activity for raw toxin ….