تاثير السبايرولينا ضد شيخوخة الجهاز القلبي الوعائي المستحدثة بواسطة الديكالكتوز في ذكور الأرانب

The effect of Spirulina spp.on the cardiovascular system aging induce by D-galactose on male rabbits

Abstract
The study is performed during the period from17 of December 2021 to 17 of January 2022 and it was designed to investigate the role of Spirulina on heart and aorta damage induced by D-galactose (D-gal) in male rabbits . Some cardiac biomarkers were estimated in the serum protein cardiac biomarkers Endothelin 1(ET1),cardiac troponine I(cTnI) and Nitric oxide (NO),some oxidant peroxynitrite (ONOO) and malonydialdehyde (MDA) and antioxidant glutathione )GSH) concentration and Electrocardiograph waves (P, QRS and T) and ST ,QT interval were monitored and histopathological examination of heart and aorta tissues were performed.
Twenty healthy adult male rabbits,weighting (1100 -1500 gm), randomly and equally divided into four groups(5/group ), normal as control group GI ,animals were received 150 mg/kg BW S/C daily of D-galactose as GII, animals were intubated 500mg/kg BW orally of Spirulina as GIII while GIV group was 150 mg/kg BW daily S/C of D-galactose with 500mg/kg BW orally of Spirulina for four weeks. blood samples were collected by cardiac punctures technique at 4 weeks of experimental study to evaluate the possibility of recovery of heart and aorta tissue damage induced by D-galactose.
The result of our study indicates a significant (p≤0.05)increase in the serum levels of ONOO, MDA with a significant increase in the serum level of Cardiac troponin I and Endothelin-1 in animal group received D-galactose in addition the results revealed a significant (p≤0.05) decrease in the serum level concentration of GSH and NO.
The results of electrocardiogram records the current study showed the prolongation in the waves ( P,QRS,T) and (ST,QT(intervals in the GII group compared to control and GIII,GIV groups .
Oral intubation of 500mg/kg B.W daily of Spirulina with D-galactose in group GIV .The results showed an improvement recovery in heart and aorta finding through a significant(p≤0.05) decrease in the serum level of the ONOO and MDA conc. ,it also showed a significant increase(P≤0.05) in the serum level of GSH and NO conc. compared to GI,GII groups .
The present study result showed a significant (p≤0.05)decrease in the serum Endothelin-1 and Troponin-I in GIII and GIV compared with another groups .The recovery role of the Spirulina in the electrocardiograph in the GIV showed a significant(p≤0.05)decrease in the (P, QRS,T)waves and )ST,QT) intervals compared to GII group.
The histological change examination of heart and aorta in GII received D-gal showed necrosis , inflammation and infiltration of inflammatory cell, and congestion ,on the other hand thickening in aorta compared with other group and study shown protective role of Spirulina in heart and aorta in GIV.
In conclusion , our study was conducted the injuries induced by D-gal ,it should be avoided especially in heart and aorta disorder from excess D-gal which can be fortified with orally intubation of 500mg/kg B.W of Spirulina to recover its adverse effects .