تحديد بعض التغييرات الكيموحيوية والمرضية النسيجية للكبد و الكلية المستحثه السمنه بالتغذيه في ذكور الفئران البيضاء المعالجه بالروزوفاستاتين

Investigation of some Biochemical and histopathological changes in liver and kidney of diet induced obesity in male albino mice treated with rosuvastatin

ِSummary

The study was designed to investigate the biochemical and histological changes in mice that consuming diet rich with animal and vegetable fats that caused obesity, which treated with rosuvastatin in its two forms (free and nano) after induction obesity for one months (the total period of experiment for four months). For this purpose about 70 Albino mice were divided into seven equal experimental groups each of 10 animals. The first group fed on normal chow diet, it was called normal diet group, second and third groups supplied by high fat diet (HFD) which consists of 15% of animal and vegetable fats. Mice treated groups with 0.02mg/kg/day of rosuvastatin and nano rosuvastatin which proceed along with high fat diet for one month, include four groups (animal high fat diet and rosuvastatin, animal high fat diet and nano rosuvastatin, vegetable high fat diet and rosuvastatin, vegetable high fat diet and nano rosuvaststin). At the end of experiment period which continued for four months all mice were weighted and sacrificed and the following assays were performed which consist:: measurement of body weight (once monthly), biochemical assay to measurement the Total cholesterol(TC), Triglycerides(TG), High density lipoprotein(HDL), Low density lipoprotein(LDL) and Very low density lipoprotein(VLDL), As well as measuring liver enzymes levels Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST), and histological study of the histopathological changes evaluation which correlated with obesity and rosuvastatin treatment, the histological study includes of the following organs: liver and kidney. the result showed: There was significant increase in the final body weight (p<0.05) in the positive control groups (animal and vegetable high fats diet groups) as compared with negative control group, but mice consuming diet rich with vegetable fat caused significant increase (p<0.05) in the body weight as compared with animal high fat diet groups. The results showed significant increase (p<0.05) in the level of TC, TG, LDL-C, VLDL-C, AST and ALT in positive control groups (animal and vegetable high fats diet groups) as compared with negative control group. TC, LDL-C and ALT enzyme levels were significantly (P<0.05) higher in vegetable high fat diet supplemented group comparatively with animal high fat diet group , while TG , VLDL-C and AST enzyme level were higher in animal high fat diet supplemented group comparatively with vegetable high fat diet group. Mice consuming high fat diet showed significant decrease (p<0.05) in HDL-C level in both positive control groups (animal and vegetable high fats diet groups) as compared with negative control group. Treated with rosuvastatin showed no significant change in the body weight in positive control groups as compared with negative control group, while treated with nano rosuvastatin showed significant reduction (p<0.05) in the body weight of high fat diet groups compare with negative control group. Mice treated with rosuvastatin and nano rosuvastatin showed significant decrease (p<0.05) in the level of TC, TG, LDL-C, VLDL-C, ALT and AST in all treatment groups as compared with positive control groups (animal and vegetable high fat diet groups) and significant increase in HDL-C, but treated with nano Rosuvastatin showed significant decrease (p<0.05), in lipid profile level and liver enzyme level compare with rosuvastatin. Changes in liver sections of mice consuming high fat diet (animal and vegetable fat) showed, mild to severe lymphocytes infiltration, sever lipid accumulation as macro and micro vesicular steatosis, necrosis, pyknotic nuclei. These changes were more sever in high animal fat liver section comparatively with liver section of mice consuming diet rich with vegetable fat. Liver section treated with rosuvastatin showed change that was of less severity as compared with positive control mice these changes included: little infiltrate lymphocytes, pyknotic nuclei, and apoptotic cells, also rosuvastatin treatment showed ameliorated the steatosis, compare with high fat diet liver section. Liver section treated with nano rosuvastatin showed much more improvement compare with liver section treated with Rosuvastatin. Kidney sections of positive control groups showed different histological changes, compared with negative control group these changes include: dilated in renal tubules, degeneration and necrosis in the cells lined renal tubules, abundant vascular congestion with thickening of the basement membrane, interstitial severe lymphocytes infiltration and widened interstitial space. Kidney section treated with rosuvastatin and nano rosuvaststin showed mild changes which include: congestion mild inflammatory lymphocytes infiltration, mild hyaline cast, necrosis, also the treated section showed normal architecture which described previously, normal glomeruli, normal capsular space and normal renal tubules.