تحضيروتشخيص بوليمر نانوي ودراسة تأثيره على بعض المعايير الكيموحيوية والنسجية في ذكور الجرذان البيض

Preparation and characterization of a nanopolymer and study of its effect on some biochemical and histological parameters in albino male rats

Abstract:
This study included two main topics:
The first: Preparation of the new nano-copolymer
The novel nano co-polymer was prepared, using the solubility process by condensation polymerization, where we added 2 moles of glycerol with 3.0 moles of phthalic anhydride at 110°C and 15 min., with water withdrawn as a by-product. The prepared nano polymer was characterized by techniques (FT-IR, 1HNMR, AFM, XRD and TEM). Then the drugs Mefenamic acid and Naproxen were added, each of them in equal proportions, in order to prepare two compounds of the nano co-polymer- drug.
The second : Study of the Biochemical Parameters and the Effects of the Prepared Nano Medicine on Rats
This study used 25 animals white male rats and divided randomly into five groups. Fist group given NaCl as physiological saline via oral rout, and consider as control group. the second and third group, they dosed mefenamic acid and the nano-polymer of mefenamic acid at a concentration of 51 mg/kg, respectively, for 14 days. The fourth and fifth groups given naproxen and nano-polymer of naproxen at a concentration of 51 mg/kg, respectively, for 14 days. The experiment to measure the biochemical parameters Aspartate transaminase (AST), alanine transaminase (ALT) and Alkaline phosphatase (ALP) in addition to measuring the concentration as well as measuring urea and creatinine as well as taking tissue sections of the liver and kidneys for the purpose of studying histological changes, the results of the current study showed:
1- The results of the study showed that the mefenamic acid and nano-mefenamic acid group had a significant increase (P<0.05) in the mean level of AST, ALT, ALP compared to the control group. The study also showed a significant increase (P<0.05) in the mean level of ALT, AST, ALP in the Naproxen and Nano-Naproxen group compared to the control group. Also, when comparing Naproxen with Mefenamic acid, we noticed a significant increase (P<0.05). In the mean ALT level of the Naproxen group, as well as when comparing the nano-Naproxen with the nano-Mefenamic acid, we notice a significant increase (P<0.05) in the Nano-Naproxen group. The study showed a significant increase (P<0.05) in the mean ALP level of the Mefenamic acid group compared with the Mefenamic acid Nano-group.
2- The study results showed that there was a significant increase (P<0.05) in the mean creatinine level in the group of Mefenamic acid and Nano-Mefenamic acid group compared to the control group, and a significant increase (P<0.05) when compared to the group of Nano-Mefenamic acid. -Mefenamic acid. There was a significant (P < 0.05) increase in the mean creatinine level in the Naproxen and Nano-Naproxen group compared with the control group, and there was also a significant (P < 0.05) increase in the creatinine level in the Nano-Naproxen group when compared to the Naproxen group. It was noticeable when comparing the group. Naproxen in the Mefenamic acid group found that there was a significant increase (P<0.05) in the creatinine level of the Naproxen group. There was also a significant (P<0.05) increase in the urea level of the Nano-Mefenamic acid group compared to the control group. The study also indicated that there was a significant increase (P<.0.05) in the level of urea in the Naproxen group and the Nano-Naproxen group compared to the control group. It was also found that there was a significant increase (P<.0.05) in the urea level of the Nano-Mefenamic acid group compared to the Nano-Naproxen group.
3- The results of the study showed that the transverse histological sections of the liver of male rats that were given drugs and nano-loaders, we observe central vein congestion, necrosis blood veins, enlargement of the sinusoids, a thickening of the nuclei, the presence of chronic inflammatory cells around the vein, and irregular cords. hepatocellular carcinoma compared with the control group.
4- The results of the study showed to the transverse histological sections of the kidney of male rats that were given drugs and nano-loaders, in which we notice severe atrophy in the size of the glomerulus and Bowman’s capsule and the destruction of the walls of the proximal urinary tubule and the distal urinary tubule and a significant increase in the size of Bowman’s space and the presence of blood congestion compared with the control group.
We conclude from the current study is the effect of the two drugs and the prepared nanocomposites on some biochemical and histological parameters in white male rats.