تحضير وتشخيص بوليمر مشترك نانوي جديد كناقل للدواء ودراسة قابليته على تثبيط انتشار سرطان الثدي

Synthesis and Characterization of a Novel Nano Co-Polymer as Drug Delivery and Studying Its Ability to Inhibit the Spread of Breast Cancer

Abstract

In this work, a novel nano particle graft co-polymer was synthesized using solubilization process by condensation polymerization from the reaction of one mole of glycerol with 2.5 moles of phthalic anhydride at 124°C in 64 Min. with the release of water as by-product, as showed in the Equation below. The product, nano co-polymer was identified by (FT-IR, 1HNMR, DSC, AFM, XRD and TEM). Synthesis several nano co-polymer-drugs by using different drug (Amoxicillin, Cephalexin, Ampicillin, Mefenamic acid, Ciprofloxacin, Pseudoephedrine, Theophylline and 4-Aminoantipyrine) respectively, by two different methods as shown in following scheme: The solubility behavior and swelling ratio was studied for all the synthesis of nano graft co-polymer-drug samples in three different media pH (2.2, 7.0 and 8.0) at constant temperature 310K as function of time (hour and day). Release of drugs (Abs.) was measured by using UV-Vis spectrophotometer in three different media pH (2.2, 7.0 and 8.0) at constant temperature 310K as function of time (hour and day). The effect of drugs loaded on the nano co-polymer (Z3, Z4, Z5, Z6, Z8, Z9, Z10, Z11, Z12 and Z13) on inhibition of the spread of breast cancer was measured, and the results showed that the drugs had a different effect, as they varied between a high effect and a low effect. IC50 value was significantly decreased in Z8 (IC50=12.64) in comparison with pure drugs and induced an apoptotic cell death pathway. The effectiveness the synthesis nano graft co-polymer-drugs in inhibiting the spread of breast cancer was in the following order: Z8 > Z6 > Z9 > Z12 > Z10 > Z3 > Z13 > Z11 > Z4 > Z5 Increasing efficacy