تحضير وتشخيص متراكبات نانوية دوائية جديدة للكيتوسان ودراسة قابليتها على تثبيط انتشار مرض سرطان القولون

Synthesis and Characterization of Nano Drug Composites of Chitosan and Studying their Ability to Inhibit the Spread of Colon Cancer

In current work to Nano-Chitosan polymer linked with drugs (Cephalexin A1, Paracetamol A2, and Mefenamic acid A3) via condensation polymerization and character its by FT-IR, 1H-NMR techniques.
Its physical properties were studied, including the solubility of drugs loaded on nanochitosan in different solvents (H2O), acetone, ethanol, hexane, DMSO, chloroform, and coloring.
The absorbance of drug release (Abs) was measured using a spectrophotometer (UV-Vis) in four different pH solutions (pH=2.2, 6.0, 7.2, 8.0) and at a constant temperature (37oC) as a function of time (hour and day).
The molecular docking of Nanochitosan- drugs were studied using the program (PyRx, Biovia), to determine the nature of the association between the prepared nanodrugs and the chain of amino acids that make up the 3m11 protein that stimulates the growth and spread of colon cancer in the HCT-29 cell line. And knowledge of the pharmacological effectiveness of chitosan nanomedicines
The effect of drugs loaded on chitosan nano polymer (A1, A2, A3,) was measured. In inhibiting the spread of colon cancer, the gene expression of the p53 gene was measured before and after exposure of the colon cancer cell line (HCT-29) to chitosan nanodrugs. The results also showed an increase in the level of gene expression when the colon cancer cell line (HCT-29) was treated with the drugs. The highest level was in the drug (A1). It was observed that the p53 gene began to function normally and began to stop abnormal cell divisions. The results showed that the drugs have an effective effect in inhibiting the spread of cancer cells. The results of the current study showed a low toxicity value of the drug (A1) compared to other drugs. The IC50 of nano-loaded chitosan drugs in preventing the spread of colon cancer was in the following order:
A1 > A3 > A2
Increased inhibition efficiency at IC50
The results also showed increased levels of production of reactive oxygen species (ROS) when treating the colon cancer cell line (HCT-29) with drugs, as the highest level was in the drug (A1).