تحضير وتشخيص متراكبات نانوية دوائية ودراسة قابليتها على تثبيط سرطان الكبد

Synthesis and Characterization of Nano Graft Copolymer Drugs and study their Ability to Inhibit Liver Cancer

In current study the synthesized of a new Nano graft Co-polymer, one mole of glycerol was reacted with three moles of phthalic acid at 130 °C in 55 min, as shown in the equation below nano graft go-polymer was then characterized using (FT-IR, 1H-NMR, AFM, and XRD) techniques.

Nano Graft Co-polymer was linked with some common drugs are (Amoxicillin, 4-aminoantipyrane, Cefotaxime, and Ceftriaxone) respectively as shown equation, The Nano Graft Co-polymer- Drugs was characterization by (FT-IR, 1H-NMR, 13C-NMR) techniques
The solubility properties of Nano Graft Co-polymer bonded with some drugs in different solvents were studied (H2O, Ethanol, Methanol, Hexane, DMSO, and Acetone).
Using a UV-Vis spectrophotometer, the release of Drug (Abs.) was monitored as a function of time (hours and days) at various pH values (2.2, 6.0, 7.2, and 8.0) at a constant 37 °C.

The most efficient binding energy of nano Graft Co-polymer drugs, the kind and length of Nano Graft Drug Composites, the prediction of protein-bonding interactions, and the preparation of the target protein’s active site were all studied in relation to the binding of medications to the proteins of liver cancer cells.
The effects of the composite drugs listed above on the liver cancer cell line were investigated. Results from our data showed promising outcomes when a number of well-known drugs and nano graft composites were combined. The rationale behind this is that the medication needs to keep working for a substantial period of time until it more effectively targets particular body parts while avoiding negative effects on other body parts. Indicating that the drug-infused synthetic nano graft composites were highly biologically successful in preventing liver cancer from rapidly spreading throughout the cells, the percentage of these cells dramatically dropped. Additionally, this sequence illustrates how well the manufactured nano graft co-polymer-drugs work to stop the spread of liver cancer:
S3 > S4 > S6> S5