تصنيع مستشعر حيوي باستخدام الألياف البصرية

Manu Factoring Of Biosensor using Optical Fiber

Abstract

This work describes the manufacturing of a biosensor. Graded index optical fiber was used along with Surface Plasmon Resonance (SPR). The manufacturer process included many steps: starting from the cutting process, then cleaning the fiber, after that the chemical aching and finally the mechanical connection to obtain the desired optical sensor. A 40 nm golden layer was deposited on the optical fiber to prepare the plasmonic surface.
Dilute hydrochloric acid at a ratio 1:10 used to ach the fiber (chemical aching) in order to obtain the following diameters (125, 105, 97, 95, 92, and 90) µm. Atomic force microscope used to measure the thickness of the diameters. After that the sensor used to detect the presence of uric acid at concentrations (4, 6, 8, 10, 20, 30, 40, 50, and 60) mM as a bench mark for the work of the sensor. Surface plasmon resonance spectrum was measured at all uric acid concentrations and the surface plasmon resonance response curve was recorded. Various sensor parameters: sensitivity, signal-to-noise ratio, analytical accuracy and efficiency value were measured for all samples and for various measurements. The highest sensitivity of 332.30 nm/RIU obtained at the resonance wavelength 443.2 nm when the refractive index was 1.3337 for the sample 60 mM when the aching time was 15 min. Furthermore, the analytical power was measured 0.833 at this sample, as well as the efficiency value that we obtained was 2.76, and the percentage of recorded, whereas the signal to noise ratio is 3.68.
The experimental results showed an increasing shift in the trough of the plasmonic resonance wavelength spectrum with an increase in the concentrations of the samples as a result of changing the refractive indexes of the medium of the mineral substance as a result of the interaction between plasmonic metal and uric acid. The results also showed a decrease in the optical fiber diameter leading to an increase in the sensitivity of the sensor.