تعدد الأشكال الوراثي لجينات GSTM1 و GSTT1 و P53codon72 في مرضى سرطان البروستات

Genetic polymorphism of the GSTM1, GSTT1 and p53 codon72 genes in prostate cancer patients.

Summary:
The role of the current study in detecting polymorphisms in the GSTM1, GSTT1 and P53codon72 genes, which are genes that have morphological polymorphisms and have an important role in the occurrence of diseases, especially cancers such as prostate cancer, samples for the study were collected from 50 patients with prostate cancer from the auditors of the Imam Hussein Center for cancerous tumors and blood diseases in Al-Hussein Teaching Hospital-Holy Karbala Governorate After diagnosis by the specialized surgeon and 50 phenotypically healthy individuals as a control group, the effect of some risk factors such as age, place of residence, smoking and family history of disease was studied in order to identify their effect On the incidence of prostate cancer, the results of the current study indicated that there are different relationships between the studied risk factors and the incidence of prostate cancer, as it showed a highly statistically significant relationship for family history of the disease, smoking and age with prostate cancer, while there was no significant relationship between the incidence of the disease and the place of residence (rural and urban), the analysis of prostate-specific antigen (PSA) was studied for both patient and control groups to show Its importance in diagnosing the disease, as it is one of the common diagnostic methods in detecting prostate cancer, as it was found that the levels of PSA were significantly higher in patients compared to the healthy group, and with high significance. The (DNA) was extracted from the blood of both study groups, patients and all healthy subjects, and molecular detection of nucleotide polymorphism in the study genes was performed using (Multiplex- PCR and PCR-RFLP) The results of molecular detection in the GSTT1 gene showed a highly significant correlation At the level of significance P ≤ 0.01 and P ≤ 0.05 between its genotypes and between prostate cancer patients and healthy subjects. , While the results did not show significant differences between the genotypes of the GSTM1 gene and between prostate cancer patients and healthy subjects, and the results did not show significant differences between the genotypes of the two GSTM1 and GSTT1 genes combined with prostate cancer patients and healthy subjects, as for the p53codon 72 gene, the results showed significant differences between the genotype (Pro-Pro) and between the two groups of patients and healthy people, as well as the genotype (Arg-Arg). While there was no significant relationship between the genotype (Arg-Pro) and between the two groups of patients and healthy people, the results also showed highly significant differences. There was a significant difference between the three genotypes (Arg/Arg, Arg/Pro, Pro/Pro) and between the two groups of patients and healthy people. The results also showed a great discrepancy between the relationship of the studied risk factors with the polymorphisms of the study genes. The results showed a significant correlation between the polymorphism of most of the study genes with All of the family history, smoking and age, while the polymorphism did not show a correlation with the place of residence for most of the genotypes of the study genes.