تقييم بروتين Galectin-3 الملزم وبروتين الصدمة الحرارية 70 وعلاقتهما بالعلامات الحيوية المضادة للأكسدة في عينة من الرجال العراقيين

Assessment of Galectin-3 Binding Protein and Heat Shock Protein 70 and Their Relations with Antioxidant Biomarkers in a Sample of Iraqi Men

It is clear that the integrity of sperm is critical for the correct transfer of genetic materials to future generations and the integrity of the sperm is responsible for fertilization. The reactive oxygen species are important signalling molecules in physiological processes, perhaps it also plays a role in pathological processes involving the reproductive system and reproduction if present in excessive quantities. Antioxidants are chemicals that lessen or prevent the effects of free radicals, thereby reducing their reactivity. Measurement of antioxidant indices of semen is used in the evaluation and the detection of the biochemical causes of male infertility.
The main purpose of this study was to identify Biomarkers that aid in the diagnosis and monitoring of male reproductive capacity. this study performed The antioxidant assessment in seminal plasma, heat shock protein 70 and Galectin-3 binding protein, clinical status, and the lifestyle of people with reproductive problems.
A case control study was conducted on 119 semen samples, 31men with asthenozoospermia, 28 men with oligoasthenozoospermia, while another 60 healthy controls. Semen samples were collected from Iraqi patients who attended infertility clinics for infertility diagnosis and assisted reproductive technologies in Karbala for the period between November 2021 and May 2022.
All participants’ questionnaire responses were put on a data sheet and given a serial identity number. Errors were prevented by using multiple entries. The Statistical Package for the Social Sciences, version 28.0, was used to create the data analysis for this project (IBM, SPSS, Chicago, Illinois, USA). On the participant data from each group, descriptive statistics were run. Mean SD was used to illustrate values for continuous variables. The Shapiro test was used to examine the data distribution. Odds ratios (ORs) and a 95% Confidence Interval Range, which were computed using a no conditional logistic regression, were used to estimate the connection between the components that had been examined. Analytical statistical tests were used to confirm that there were significant variations in categorical variables between the parameters. Results of every hypothesis test with a two-sided p-value of 0.05 or lower were deemed statistically significant
The average semen total antioxidant level showed the highest level in the control group (3.53±0.64U/ml), and the lowest level was associated with oligoasthenozoospermia (1.88 ± 0.15 ng/l). The results of this study also showed that the average level of heat shock protein 70, the lowest, was observed in the case of the control group (40.17±1.94 ng/L) and the highest level was associated with a decrease in oligoasthenozoospermia. 50.02 ± 7.39 ng/L). As for the average rate of Galectin-3 binding protein level, it was not statistically significant between the control group and Asthenozoospermea and Oligoasthenozoospermia, Heat shock protein 70 was associated with the total redox state of semen significant inverse correlation
There is a correlation between heat shock protein 70 and total semen antioxidants, and this indicates the importance of measuring each of them, in cases of infertility and not being satisfied with one over the other.