تقييم كفاءة المركب المكون من اوكسيد الزنك والمركب Triclosan في تثبيط عدد من العزلات السريرية لخميرة البيضاء المبيضة

Evalution efficiency of composite containing ZnO/Triclosan for inhibiting of numerous of clinical isolates of Candida albicans

ABSTRACT

This study included isolating the yeasts that cause oral thrush in children (Candid albicans), diagnosing them using traditional and molecular methods, and identifying some of their virulence factors represented by the production of phospholipase, their ability to degrade blood, and their ability to produce biofilm.
In addition to preparing and identifying a hybrid compound of the compound (Triclosan) and evaluating its inhibitory effectiveness against the yeasts isolated in this study, the results showed the presence of 23 isolates of C. albicans yeast from a total of 120 samples taken from children after identifying the yeasts and growing them on HiCrome differential medium. ™ Candida Differential Agar. The results of molecular diagnosis using primers (CALB1, which is specific for C.albicans yeast, with a size of bp 273, and ALS1 and HWP1, with a size of bp 318 and bp 572, which are specific for biofilm formation in yeast, showed the presence of the three genes by 100% in the twenty-three isolates tested.
The results of examination by infrared spectroscopy (FT-IR) showed a shift in the frequencies of some functional groups towards high and low frequencies. X-ray spectroscopy (XRD) also revealed the appearance of new diffraction levels in the spectrum of the TCS-ZnO hybrid composite compared to the spectrum of zinc oxide.
The results of examination by atomic force microscopy (AFM) showed that the average dimensional diameter of the TCS-ZnO hybrid disinfectant particles reached 108.89 nanometers.
The inhibitory activity of the free Triclosan disinfectant, as well as the hybrid compound prepared in this study, was studied against the yeast isolates obtained in this study as well. The results showed the following:
Isolate C11 was the most sensitive to the inhibitory action of the free disinfectant, with an inhibition diameter of (42.5 mm), while isolates C15, C23, and C1 were the least sensitive to the inhibitory action of the aforementioned disinfectant, with diameters of inhibition of (27-26.5-25) mm, respectively.

The results also showed that isolate C 13 is the most sensitive to the inhibitory action of the TCS-ZnO compound when using a concentration of 1024 µg/ml with an inhibition diameter of 40.5 mm, while isolates C8 and C 21 were the least affected by the inhibitory action with an inhibition diameter of 21 mm.

It also turned out that 9 isolates of C.albicans yeast were producers of the phospholipase enzyme, representing 39.1% of the C.albicans isolates obtained in this study. In addition, all of the yeast isolates above were hemolytic by producing 100% hemolysin, and all isolates have the ability to produce biofilm at 100% with strong productivity.