تقييم مستويات TLR4 و CD19 لدى مرضى التدرن باستخدام تقنية التدفق الخلوي في محافظة كربلاء المقدسة

Evaluation Levels of TLR4 and CD19 in Tuberculosis Patients using Flow Cytometry at Kerbala Holy Governorate

This study was conducted in the Center for Chest Diseases and Al-Hussein Teaching Hospital in the holy governorate of Kerbala for the period from October / 2020 to April / 2021. The study included 51 cases of both sexes, their ages ranged between (18-60) years, they were divided into two groups: The first group included 36 cases of tuberculosis patients (25 under treatment and 11 new diagnosed cases), and the second group was the healthy group, which included 15 healthy individuals of both sexes who do not suffer from any chronic or inflammatory diseases.
The study aimed to evaluate the effect of both TLR4 and CD19 immune standards on the immune response in Tuberculosis patients. Tests were performed using flow Cytometry technology, as well as an Interferon-Gamma release assay (IGRA) test using ELISA technology for the new diagnosis group (new infection). To confirm infection.
The results of the study indicated an increase in the number of cases of tuberculosis among the age groups 21-30 years and 31-40 years, which included 17 patients (47.2%) and 8 patients (22.2%).
The results of the current study showed that the incidence of male tuberculosis is higher than that of females, as the rate of male infection was (52.8%) and the percentage of female infection was (47.2%).
As for the results of the statistical analyzes related to measuring the level of TLR4, it showed that there were significant statistically significant differences at the significance level of p ≤ 0.001 between the new diagnosis group (recent infection) and the healthy group by noting an increase in the level of TLR4 in samples of newly diagnosed tuberculosis patients.
While the statistical analysis showed an increase in the level of CD19 in newly diagnosed patients compared to the healthy group.
The results of the current study showed that there were no significant differences in the level of TLR4 and CD19 between males and females.
Also, there were no significant differences in the level of the studied immunological criteria among the studied age groups.