دراسة التأثير الوقائي والعلاجي للمستخلص الكحولي لنبات حب العزيزCyperus escule في بعض المعايير الكيموحيوية والنسجية لكلى الجرذان المصابة تجريبيا بالفشل الكلوي

The study of the preventive and therapeutic effect of alcoholic extract of Cyperus escule on some biochemical and histopathical parameters of kidneys of male rats induced renal failure

Summary

This study aimed to know the effect of the phenolic extract of Cyperus esculentus on some biochemical and histological parameters of kidneys in male rats treated with gentamicin (Gn) to induce renal failure. This study was conducted in the animal house of the College / Pharmacy / University of Karbala, during the period from September 2020 to April 2021, 72 adult male laboratory rats were used, and they were obtained from the animal house of the Department of Life Sciences / College of Science / Babylon University . Their ages ranged between 3-4 months and their average weight ranged between (200-250) grams, and these rats were divided into 6 groups, and each group included 12 rats and they were as follows: 1- Physiological solution group: The rats of this group were fed a normal saline solution during the duration of the experiment. 2- 2- Positive control group (G2): The rats of this group were injected intraperitoneally with 120 mg/kg/day gentamicin for twelve consecutive days. 3- 3- Alcoholic extract group (G3): The rats of this group were dosed orally with 170 mg/kg/day alcoholic extract of chervil for 30 consecutive days. 4- 4- Group (Gentamicin + alcoholic extract) G4: Rats of this group were injected intraperitoneally with gentamicin 120 mg/kg/day for twelve consecutive days, then they were given orally administered with 170 mg/kg/day alcoholic extract of chervil for 30 consecutive days. 5- 5- Group (alcoholic extract + gentamicin) G5: rats of this group were dosed orally with 170 mg/kg/day alcoholic extract of chervil for 30 consecutive days. Then they were injected intraperitoneally with gentamicin 120 mg/kg/day for twelve consecutive days. 6- 6- Group (Gentamicin + alcohol extract) together G6: Rats of this group were injected intraperitoneally with 120 mg/kg/day of gentamicin for twelve consecutive days and at the same time were given orally administered 170 mg/kg/day alcoholic extract of cherry blossom for 30 consecutive days. 7- Blood and nephrectomy samples were collected after completion of the experiment and blood serum was obtained for the purpose of measuring the level of the following parameters: glomerular filtration rate, neutrophils gelatinase, creatinine, urea, uric acid, albumin and antioxidants (calyase, glutathione and glutathione peroxidase) as well as histopathological study of the kidneys. group light microscopy. The results of the current study showed that the induction of chronic renal failure by gentamicin led to a significant (P≤0.05) decrease in the level of GFR and levels of antioxidants (2.38, 9.68,38.07, 213.88), respectively, and a significant increase (P≤0.05) in the level of creatinine, urea, And albumin, and gelatinase neutrophils (4.10, 80.38, 6.50, 13.62 for all concentrations, respectively, compared with the control and treatment groups. The induction of chronic renal failure led to Histological changes occurred in the kidneys of treated rats compared to the control group, as there were changes in the tissue of the kidneys that occurred with blood congestion and degeneration of the renal tubules, necrosis, glomerulitis, infiltration of inflammatory cells, the presence of protein substances in the tubules, kidneys, expansion of Bowman’s capsule, and cellular increase in the glomerulus. It was concluded from the above study that treatment with the alcoholic extract of the cherry tree has a positive effect in repairing the damage caused by gentamicin poisoning on many physiological and histological parameters and gave protection to the kidneys from harmful effects with a clear improvement of the kidney tissue by reducing the degenerative changes of the glomerulus and its structure approaching The natural form as well as increased ability to ac as antioxidant agents, as well as anti-inflammatory agents.