دراسة بعض التأثيرات الوراثية لمستخلص حبوب لقاح نحل العسل في ذكور الفئران البيض

The Study of Some Genetics effects For Pollen Grains Extract of Honey Bee in Male of White Mice

The current study was included the test of the activity of methanol water extract of pollen grains as an antioxidant and antimutagenicity. As it has been detected the harmful effects of drug and try to cyclophosphamide and try to reduce them by using this extract.

This extract has been described by using thin layer chromatography TLC . The results which showed the presence of more than one component of this extract when using multiple different liquid phases.When examining the activity of antioxidant betacarotene spray process .It was observed appearance of the antioxidant activity in several bands . The antioxidant activity was determinated for these bands through the retention of the color yellow, which indicates the existence of effective antioxidant in some components of pollen extract .

It has shown the type of electrophoresis for the DNA that extracted from white blood cells of mice. The treatment dose of 20 mg kg of the cyclophosphamide , a led to the appearance of a large smear and clear .The size of molecular ranging from 9.4-0.9  kilobase pair, which was where the level of fragmentation 8.5 kilobase pair compared with the negative control. The interaction at a dose of 5 mg kg for extract of pollen grains, it was noted that the levels of fragmentation of the DNA in the first , second and third interactions are (0 , 1.4, 8.5) kilobase pair respectively . The interaction at a dose of 15 mg kg, it was noted that the levels of fragmentation of the DNA in the first , second and third interactions are (3.1,3.8,8.5) kilobase pair respectively . The interaction at a dose of 30 mg kg, it was noted that the levels of fragmentation of the DNA in the first , second and third interactions are (4.8,5.3 8.5) kilobase pair respectively .This indicates that the interaction first, at a dose of 5 mg kg is the best in reducing the level of the crash in the DNA, followed by the second interaction, while the third interference did not cause  at all doses of any touched positive changes to prevent crash DNA.

This has shown the results of a mitotic index (MI) that the average of MI cells of the  bone marrow of mice that have been treated by doses (5, 15 and 30) mg kg of the extract of pollen grains  and the dose of 20 mg kg of the cyclophosphamide in the three interactions . It reached the values of any of the interactions or (before, with, after) the dose 5 mg kg 20.2, 18.86, 8.6 respectively, The dose of 15 mg kg are 14.1, 10.08, 8.5 respectively. It was also noticed a significant difference (P<0.01) in the value of MI when comparison with first and second interactions of the first dose of the extract of pollen grains, and the first and second of the interactions from second dose of the extracted of the extract of pollen grains with the positive control, which led to the raising of the mitotic index.

With regard to the results of chromosomal abnormalities, it was shown that the dose of 5 mg kg in the first and second interactions led to a significant difference (P<0.01) compared with positive control. While the third interaction does not lead to significant difference . But when a dose of 15 mg kg has been shown that the first and second interactions led to the significant difference (P<0.01) .While the results of the third interaction is not significant .The dose of 30 mg kg, it led to the first and second interactions significant difference (P<0.01) , while the third interaction did not lead to significant difference .

when testing for abnormalities in sperm. It turns out that the dose of 5 mg kg in the first and second interactions led to a significant difference(P<0.01)  compared with positive control , while the third interaction did not lead to significant difference . At a dose of 15 mg kg showed the first and second interactions resulted in a significant difference . While the third interaction did not lead to differences. And at a dose of 30 mg kg the first and second interaction led to occurrence of significant differences (P<0.01) . While the third interaction does not lead to significant difference .

As a result of the present study, we can conclude that a methanol water extract of pollen grains has activity antioxidant and antimutagenecity when given before and with of mutagen and low doses  in both somatic and germ cells.