دراسة بعض المعايير المناعية لدى المصابين ببكتريا Helicobacter pylori من المدخنين

Study Of Some Immunological Variations Of Smoking Patients Infected With Helicobacter pylori

Through this study was to shed more light to see the change in the levels of certain variables immune to the peoples who infected with Helicobacter pylori as well as, knowledge of the relationship between infection and smoking and their impact on Immunoglobulin’s levels ,the current study included 45 people (apparently healthy) as the control group to study serum samples were collected from a hospital-Husseini education in the province of Karbala (Endoscopy Unit) during the time period from 01.11.2010 until 01.04.2011. sera were analyzed 45 patients of infectious ulcers and 16 healthy ( control group) to detect the presence of antibodies IgG and IgM immune supplements and C3 and C4 of the bacteria H. pylori using a Signal Radial Immune Diffusion (SRID), has also been measuring the level of IL-8 ELISA technique using the Enzyme Linked Immunosorbent Assay (ELISA).

1. showed the results of the present study for low levels of immune-type Immunoglobulin’s IgG and IgM levels, as well as immunological complements C3 and C4 in patients with bacteria H. pylori gradually with age, reaching a maximum concentration of IgG rates in the age group (20-31 years), amounting to 914.14, while the moral dropped to 430.94 mg / d. L. in the age group (60-71 years) compared with the control group, which amounted to 1026.89 mg / d. L. , as well as low levels of immunoglobulin IgM gradually with age in all groups under study appeared as a moral decline in the age group (40-51 years) to 105.50 mg / d. L. until you reach the lowest rates in the age group (60-71 years) 29.94 mg / d. L., compared with the control group, amounting to 1026.89 mg / d. L. , also found a significant decrease in the concentration of immunoglobulin molecule C3 in all age groups studied and the back of the decline is evident in the age group (60-71 years) reached 49.54 mg / d. L. as compared with the control group 102.91 mg / d. L., while the back of a moral decline in the concentration of immunoglobulin molecule C4 of the age group ( 40-31) years until he reached the age group (60-71 years) 51.82 mg / d. L. , compared with the control group, which has reached 66.29 mg / d. L. , while that recorded the results for the current high rates of motor cell IL-8 is a moral in all age groups and with aging It reached 62.71 pg / ml in the age group (20-31 years) and reached maximum rates in the age group (60-71 years) to 80.20 pg / ml, compared with the control group 18.25 pg / ml .

2. The results show that smoking reduces the levels of Immunoglobulin’s directly proportional to the intensity of smoking with decreased concentration of IgG is significant with the increase in the amount of smoking in smokers low and moderately strongly to 745.44, 662.33, 695.26 mg / d. L. , respectively, compared with the control group and the average concentration of IgG with 1044 mg / d. L. , while the observed began to decline in concentration of IgM is moral in the middle-smoking and smoking strongly to reach 78.20, 76.33 mg / d. L. , respectively, compared with a control group of 110.87 mg / d. L. As for the effect of smoking on the levels of supplements immune rate has fallen molecule immune C3, is a moral to the group smoking low and in average, smokers strongly to 60.200, 55.36, 55.77 mg / d. L. , compared with the control group of 106.91 mg / d. L. , while the decline was significant in the concentration of the molecule immune C4 smokers are average, smokers strongly to reach 58.48, 52.43 mg / d. L. , compared with the control group 66.20, while the observed high concentration of IL- 8 with the intensity of smoking, with an average height and a moral to a moderate smokers and smokers strongly respectively 63.67, 69.08 pg / ml , compared with the control group, which was the rate of cellular concentration of motor 44.83 pg / ml .
3. recorded the results that each of the infection, smoking has an effect in the levels of Alglupulinat immune supplements immune as decreased IgG, IgM, C3 and C4 in patients and smokers at the same time to 672.22, 78.33, 54.06, 57.25 mg / d. L. , respectively, and that the decline was insignificant compared with the groups of control (not infected – smokers) 763.21, 97.45, 63.78, 59.86 mg / d. L. , respectively, as well, while the observed high level of IL- 8 is significant in people – smokers compared to 74.16 pg / ml with a group of control and have a 20.50 pg / ml .

In light of the demonstrated by the results we can deduce the existence of an inverse relationship between age and levels of immunoglobulin’s IgG and IgM as well as the levels of supplements immune C3 and C4 in serum was observed low concentration of these proteins immune with age, while the relationship was direct correlation between the concentration of IL-8 and the aging , the results showed the existence of a negative effect of smoking on levels of immunoglobulin’s IgG and IgM as well as supplements the immune C3 and C4 have decreased concentrations of protein in smokers with the increase in the amount of smoking until it reached its lowest level in smokers heavily, while the rates increased IL-8 in serum-smokers with the increase in the amount of smoking until you reach higher levels in smokers heavily It also, concluded that a positive relationship between the incidence of bacteria H. pylori, smoking a negative impact on the levels of immunoglobulin’s IgG and IgM as well as supplements the immune C3 and C4 as observed low levels of these proteins in the immune serum with bacteria H. pylori, smoking at the same time, while the relationship between the incidence of smoking and extrusive effect on the levels of IL-8 as it was noted the high level in serum patients and smokers.