A Molecular and Bacteriological Study of Some Strains for Providencia Isolated in Karbala

A total of 164 different samples obtained from patients admitting to AL-Hussien Hospital in Karbala Province ,only  two isolates of Providencia spp. were isolated and identified by means of traditional techniques and API system , One of the isolates was found in  urine sample and the other in a burn swab.

 The effect of some antibiotics on the two isolates was investigated, and the results showed that the isolates were resistant to Rifampicin, Ampicillin, Amoxcillin, Gentamycin, Trimethprim, Pipracillin, Clindamycin, and Cefotaxim, and sensitive only to Ciprofloxacin .

Some virulence factors of the isolates were also studied, and the results showed that the two strains have capsules which be regarded as the most virulant factor of the bacteria . Furthermore the results showed that the two strains have adhesion factors ,too .

The ability of the isolates to produce haemolysin, and  siderophores was also observed and  the results of this work showed that the isolates were unable to produce bacterial haemolysin ,but were able to produce siderophores .

The resultes of the plasmid DNA Study,  showed that Providencia have more than one plasmid. Curing techniques by using  SDS (Sodium Dodocyle Sulfate) showed that the bacterial isolates lost their plasmid content which indicates that SDS can make curing to the plasmids completely .