دراسة تأثيرالتعرض لاشعة الهاتف المحمول على بعض المعايير الوراثية الخلوية والفسيولوجية في ذكور الجرذ الأبيض

Study of effect of mobile phone exposure on some cytogenetic and physiological parameters in Albino rat

The present study was conducted to investigate the effect of long exposure to mobile radiation on antioxidant the Level of DNA damage and rate chromosomal abberation  rats.The present study was carried out in college of Karbala Labs in Faculty of pharmacy from November 2017 to April 2018.The study in volved sixty healthy male white Albino rat, the average weight (190-240)g ، and(10-12) week.The rats were random divided to the four groups , and contain for each the group on (15) rat. Three groups were exposured to mobile radiation for three perids (2-3-6) months .The first group (T1) was exposed to irradiation of the mobile phone with frequency (900) MH for two hours and for three periods (2-3-6), months the second group (T2) was exposed to mobile phone radiation for (4)hours and for three period (2-3-6) months. The third group (T3) was exposed to mobile phone radiation for eight hours and three periods of time (6-3-2) months and the four group was control group not exposed to mobile phone radiation for (2-3-6) month. Blood samples were collected from the four groups after the end of the trial period (2-3-6) months the physiological parametrs were studied : The measurement of the concentration of levels of mononaldehyde (MDA), the level of nitric oxide (NO) and the level of reduced Glotathione (GSH) and levels of Catalytic(CAT) and superoxide dismutase (SOD) in the serum. The genetic study the level of DNA decomposition and chromosomal deformation rate of bone marrow cells was measured. The results of the present study were as follows:

1-Significant increase(p≤0.05)in the concentrations of MDA and NO and significant decrease (P <0.05) in the concentrations of GSH, CAT and SOD in the serum for groups exposed to mobile phone radiation and for (2-3-6) months and a period of (8-4-2) hours respectively compared to the control group for each group.

2- DNA damage was measured by score comet assay DNA,% Tail, Tail length, and Tail Moment the results showed that the highest effect was for the last group T3 of the mentioned standards.

3-The mitotic index was estimated for three groups exposed to mobile radiation. The results showed no a significant increase(p≥0.05) for the first group T1, while significant increase (p≤0.05)for the T3 and T2 groups compared with control group.The results of the study showed chromosomal abnormalities in the bone marrow cells, which resulted in the emergence of chromosomes of the ring and chromosomes deleted and broken and chromosomes of the center and decentralized of the masses exposed to the radiology of the phone showed a significant increase (p≤0.05) of the chromosomes mentioned above . The current study concludes that long exposure to mobile radiation causes physiological changes and cellular and molecular genetic changes in white rat males.