دراسة تأثير كل من المستخلص النباتي , المستحلب النباتي والزيت الطيار لنباتي الميرمية والبردقوش في حياتية طفيلي اللشمانيا الأحشائية Leishmania donovani خارج الجسم الحي In vitro

Rp_ Study of the effect of aqueous extract, plant emulsion and volatile oil of Saliva officinalis and marjoram on the life of the visceral Lei... pdf

Summary
Visceral leishmaniasis is one of the problems facing infected patients in general and children in particular. Therefore, this study included identifying the spread of visceral leishmaniasis. The survey study was conducted in the hospitals of Karbala governorate, which included Al-Hussein Medical Hospital, Al-Hindia Hospital, Al-Hussainiya Hospital, Ain Al-Tamr Hospital, Children’s Specialist Hospital, Which was conducted for the period from January 2021 to June of the same year, and after diagnosing cases by specialized doctors and among 252 patients who underwent examination, it was confirmed that 17 of them had visceral leishmaniasis, distributed among the governorate’s hospitals, and the current study reviewed the data of the age group and gender. The areas of spread according to the geographical area and the months of infection.
As the results proved that the highest rates of infection were in the months of, April and with percentages of 0.4% respectively, with an average general rate for the months of infection amounted to 3.52%, and that The highest injuries according to the geographical area were recorded in the Children’s Specialized Hospital affiliated to the center sector of the Karbala governorate with a percentage of 8.24% with a general average rate of total injuries for the areas covered by the research 6.74%, and it was noted that the highest percentage of injuries according to the age factor amounted to 7.14% and this percentage It is for the age group limited between 9-5 years, and the results showed that male children were affected by the disease more than females, with an average general rate of 1.25%.
The cytotoxicity assay (MTT assay) was used to detect the effect of treatment with plant extracts and volatile oils of sage and marjoram on the life of the parasitic flagella Promastigots, First, 3 drug spectra were used for each plant, represented by aqueous extracts, a plant emulsion, in addition to the volatile essential oil. Second, a series of dilutions was conducted to make six concentrations for each drug spectra of the plants under study, The concentrations of aqueous extracts are 200, 100, 50, 25, 12.5, 6.25 µg/ml, and the same concentrations were used for the plant emulsions, while the concentrations of the essential oils of the two plants were as follows 1000, 500, 250, 125, 62.5, 31.25µl/ml.
The results of the cytotoxicity examination under the influence of treatment with plant extracts and essential oils of the two plants under study showed that the two highest concentrations of aqueous extract of sagebrush 100, 200 µg/ml had given the highest percentage of cellular growth inhibition with a value of 92%, 92.2%, While the aqueous extract of marjoram did not show any toxic effects on the parasitic cells, while the second concentration 100 µl/ml of sage plant emulsion showed the highest inhibitory percentage amounted to 89%, and the first concentration of marjoram emulsion gave the highest percentage of inhibition that reached 52%, as for the essential oils of the two plants, the fifth concentration 62.5 µl/ml of sagebrush oil gave the highest percentage 86%, while the first concentration of the essential oil of marjoram was 1000 µl/ml The highest percentage was 68%.