دراسة جزيئية للتحري عن نسب الاصابة بداء المقوسات الكوندية في النساء المصابات بسرطان الثدي الورمي بنوعيه الخبيث والحميد في مدينة كربلاء

Molecular Study to Investigate the Incidence of Toxoplasmosis in Women with Malignant & Benign Breast Cancer at the Holy Kerbala

Abstract

The current study aimed to compare the incidence of Toxoplasma gondii among patients with both benign & malignant breast cancer in the Holy Kerbala provice. The current study was conducted in October of From 2023 to June 2024, 150 samples of malignant breast cancer tissue embedded in paraffin wax & diagnosed by the specialist doctor were collected, & 50 samples of benign tumor tissue, These samples were collected from the tissue sectioning laboratory in Imam Hussein Medical City, the Al-Kafeel Specialized Hospital Laboratory, & the Specialized AL-Sajad laboratory for histological & cellular examinations & tumor diagnosis in Holy Kerbala.
The study samples included three groups: the first group consisted of 66 women with breast cancer who did not undergo chemotherapy, the second group consisted of 84 women with breast cancer who underwent chemotherapy, The third group consisted of 50 women with benign tumors. The ages of the patients ranged from 25 to 54, The polymerase chain reaction (PCR) technique was performed, as was identified, Most of the genotypes of the toxoplasmosis parasite were identified using a molecular technique called Restriction Fragment Length Polymorphism (Mn-PCR-RFLP) on short-str&ed RNA, DNA in tissue samples affected by breast cancer for all groups under study, based on the amplification of the B1, SAG3, & SAG1 genes, as special cutting enzymes were used to determine the genotype, For the Toxoplasma gondii parasite, the Hhal enzyme was used to determine the second genotype at 221bp, & the Sau3Al enzyme was used to determine the third genotype at 241bp, & histological examination.
The results showed that the incidence of toxoplasmosis in breast cancer patients was 11.3%, compared to 4% in women with benign tumors, & that the infection rate in women who received treatment 76.5% As for women who did not receive treatment, the infection rate reached 23.5%, & the risk of infection increases the probability of sick women developing breast cancer by 3.7% compared to healthy women, as indicated In the current study, the age group most affected by Toxoplasma gondii was the group <56, as the infection rate in this group was 37.3%, The results revealed that the most common genotypes that appeared in the study groups were the second genotype & the third genotype. However, the results of the histological examination did not show any clear histological changes in the tissues of the study groups.