دراسة جزيئية ومناعية للنساء ‏المصابات بالتهاب المفاصل الرثياني ‏في محافظة كربلاء ‏

‎ Molecular and Immunological study of women ‎with rheumatoid arthritis in karbala province ‎

The current study included the collection  of   (44) an a blood samples from patients with rheumatoid arthritis and non-treated  from female and 22 blood samples of healthy people  from Karbala governorate and the patients of the Imam Hussein Medical Hospital in the consulting clinic for the patients of the joints of females with ages ranging from (20-60) years and from January 2018 to May 2018, where patients were diagnosed by the consultant doctors at Imam Hussein Medical Hospital.

        The study included two aspects that included the first aspect of the molecular study by  the technique of the interact PCR ( Polymerase Chain Reaction)  that we has release the percentage of gene in patients samples was 98% and the healthy samples 100%, The molecular description  which by using the PCR technique  for the gene that include in study  and its name is (Single transducer and activator of transcription4) STAT4 in patients with rheumatoid arthritis , and also user another technique for the molecular description and its also depend on technique of (PCR) that name is   RFLP(Restriction fragment length polymorphism) to know the polymorphism for the gene STAT4. Three genotypes were identified GG  and GT genotype and the TT genotype in patients with rheumatoid arthritis and the healthy group. The percentage  of GT genotype it’s the upper most in patients and the next type  GG and the  TT was the lowest  genotype the in rheumatoid arthritis patients, The study also revealed that age and weight gain were associated with an increase in the incidence of the disease, as well as a higher incidence of the disease in patients with a previous family history of infection and increased incidence of the disease in smokers and increased the incidence of disease in patients with diabetes The study reported a high incidence of high blood pressure in patients with rheumatoid arthritis. The immunological study included measuring the level of three cytokines, interleukin-1 beta, interleukin 4 and interferon gama.

           The Immunological study  Apparently The results of the immunological tests is being high in  focus standard the immunoglobulin  cytokine IL-4  high morally (P≤0.05) to the people who was casualty with RA compare with healthy group.

The immunological tests is being  release a high concentricity standard the intraleukin-1 beta and IFN-Y  high morally (P≤0.05) for the people who was sick with RA  compare  with healthy group.

          The study also found that there is a correlation between geneotype of the STAT4 and some immunological parameters, noting that the level of the interleukin 4-type cytokine in the group GT high compared to the TT and GG. The high level of 1-beta and IFN-Y  cytokine was observed in the GT-group compared with the rest of the genotype.