دراسة دور تعدد الاشكال الجيني للانترلوكين 17-A ومستوى المصل في النساء اللواتي يعانين من متلازمة تكيس المبايض

Rp-Study The Role of Interleukin 17A Gene Polymorphism and Serum Level in Women with Polycystic Ovarian Syndrome.pdf

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder among women of reproductive age, characterized by menstrual abnormalities and clinical or biochemical features of hyperandrogenism. PCOS is associated with multiple comorbidities, including obesity, insulin resistance and type 2 diabetes, mood disorders, Obstructive sleep apnoea (OSA) and cardiovascular disease (CVD). The current study has aimed at determining the influences of genetic variants of interleukin-17A (rs2275913) polymorphism on Polycystic ovary syndrome (PCOS) in addition to ELISA detection of serum IL-17A level.
This study is a case-control study, and the subjects of this study are divided into two groups control (n=50) and patients (n=50) enrolled in Najaf province , AL-Sader Teaching Medical City and AL-Zahraa Teaching Hospital during the period from November 2021 to April 2022.
The samples of blood were collected from each participant, noting that the sera were used to determine serum IL-17A level for all samples by ELISA technique. In contrast, whole blood was used to determine white blood cell and lymphocyte count and DNA extraction. Using Sysmex XN-350, five differential automated hematology analyzers are used to detect white blood cells and lymphocyte count. Moreover, detect IL-17A (rs2275913) gene polymorphism by Real-Time Polymerase Chain Reaction (RT-PCR) technique. MINI VIDAS detects the levels of luteinizing hormone, follicle-stimulating hormone, thyroid stimulating hormone and prolactin hormone.
This study has showed a significant association (P=0.005) between IL-17A serum level and PCOS patients compared with healthy control. The mean of IL-17A in patients was (11.46), and the mean of healthy control was (9.53). Regarding WCC and lymphocyte counts, there was a nonsignificant association between PCOS patients and healthy control with (P=0.230) for WCC and (P=0.231) for lymphocytes. About the genetic polymorphism of the IL-17A gene (rs2275913), the current study demonstrated that the AG genotype was found to be at a higher frequency in patients compared with control (50.0% vs. 42.0%, respectively), the GG genotype was found to be at a higher frequency in control compared with patients (46.0% vs. 34.0%, respectively) and the AA genotype was elevated in patients compared with control (16.0% vs. 12.0%, respectively). In the genotypes, there was no significant association between the studied groups (P=0.421). Also, in the alleles frequency, there were no significant differences between the studied groups (P=0.307). In addition, this study found a nonsignificant association of IL-17A serum level with genotypes and alleles frequency of IL-17A gene (rs2275913) polymorphism in PCOS patients.
This study demonstrated that the IL-17A gene (rs2275913) polymorphism was non significant associatied with PCOS. therefore, might have a protective role in PCOS patients. In addition, the IL-17A may be used as a predictive marker for diagnosing PCOS.