دراسة سمية الحديد،والتحلل التأكسدي للدهون وموت الخاليا نوع Ferroptosis لدى مرضى الزهايمر والشلل الرعاشي

Iron Toxicity, Lipid Peroxidation and Ferroptosis among Patient with Alzheimer and Parkinson Diseases

Neurodegenerative diseases (NDDs) are age related conditions described by
uncontrolled neuronal death leading to a progressive degeneration in brain functions.
NDDs affect millions of people worldwide. Alzheimer’s disease and Parkinson’s
disease are the most common of NDDs. These diseases related to complex and
intricate cell death mechanisms and multiple pathways, which include excessive
accumulation of iron and lipid peroxidation in different brain regions. The most
common NDDs include Alzheimer’s disease(AD),Parkinson’sdisease(PD),prion
disease, Amyotrophic lateral sclerosis(ALS), Huntington’s disease(HD), spinal
muscular atrophy, and spinocerebellar ataxia.
Ferroptosis is a type of programmed cell death dependent on iron and characterized
by the accumulation of lipid peroxides,it is genetically and biochemically distinct
from other forms of regulated cell death such as apoptosis. ferroptosis initiated by the
failure of the glutathione-dependent antioxidant defenses, resulting in lipid
peroxidation and eventual cell death.
This study aimed to evaluate the role of free iron, their transported such divalent
metaltransporter1(DMT1), Totalironbindingcapacity(TIBC), 5-lipoxygenase(5LOX)
,Malondialdehyde(MDA)and ferritin level in Alzheimer disease and Parkinson
disease .
A case control study ,80 samples were collected from 40 cases
(24)Alzheimer’sdisease(AD)frome psychiatry clinic,(16) Parkinson’s disease
(PD)frome Neurology clinic aged between (65-90) years and 40 participants as a
healthy control with age range (65-87).
Examination of serum levels of DMT1, MDA, 5LOX were measured using enzyme
linked immunoassay (ELISA) technique. serum levels of biochemical tests (lipid
II
profile, free iron, ferritin, TIBC) were performed using a clinical chemistry analyzer,
Complete blood count was done by XP-300™ Automated hematology analyzer
Sysmex.
Results indicated that, patients with neurodegenerative disease showned significant
increasing in the range level of the TIBC, MDA, Free Iron and Ferritin when
comparing to the healthy control groups (p value were <0.05), while the range level
of 5LOX and DMT1 were decreased compared to healthy control and only 5LOX was
shown a significant differences (p value were <0.05). ROC curve and AUC analysis
for the biomarkers were performed. Increased level of Iron showned good
performance for prediction Neurodegenerative disease, TIBC was the best prediction
for Alzheimer’s and Parkinson’s disease cases.
It was concluded that, iron forms were linked successfully in the diagnosis of
neurodegenerative disease. It is likely that iron’s defective homoeostasis and their
redox-activity plays a role in the neuropathology of Alzheimer’s and Parkinson’s
disease