دراسة في بعض التغيرات الهرمونية والمعايير الفسيولوجية لمرضى عدم الخصوبة

Study of some hormonal changes and physiological parameters of infertile patients

This study aims at investigating the hormonal levels and parameters of sperms changes in some infertile patients in Kerbala. The levels of inteinizing hormone (LH), prolactine hormone (PRL), lestosterone (T) mac roscopoc and microscopic examination of seminal flnid have been measured. Part of this work was done in the laboratories of Kerbala where the samples was collected, while the other part was done in the laboratories of Biology department, college of Education Kerbala university where the hormonal analysis were done.

Blood samples were collected from 79 infertile men; all of them lived in Kerbala. There were different cases of infertility including. 37 cases with asthenospermia, 22 cases with Oligospermia, 20 cases with azoospermia. We also have taken 19 fertile men (they have children) as a control in the study to correlate achange in the data studying. 4 ml of blood were withdrawn by sterile syringe, the sera were separated by centrifugation at 3000 round/ minute. Then the levels oh hormones were measured using “Enzyme linked immunosorbrnt assay” (ELISA) for hormonal analysis, the results could be summarized as follows:

Measured of levels hormones was appeared there was significant increase (P>0.05) in the levels of luitinizing hormone in Oligospermia patients, while there were no significant differences in the levels of hormone in the asthenospermia and Azoospermia. There was significant increase (P<0.05) in the levels of prolaction hormone in Oligospermia and azoospermia patients, while there were no significant differences in the levels of the hormone in asthenospermia also there was significant decrease (P<0.05) in the levels of testosterone in asthenospermia and azoospermia while there were no significant differences in the levels of that hormone in oligospermia.

Result of macroscopic examination was appeared there was significant decrease (P<0.05) in the volume mean of seminal fluid in all taken in fertile men were of seminal fluid in all taken in fertile men as compared with control group while there was significant increase in PH mean in all taken in fertile men as compared with control group.

Results microscopic examination appeared there was significant decrease (P<0.05) in the concentration of sperms in olligospermia and azoospermia and there was no significant changes in asthenospermia as compared with control group, the results appeared there was significant decrease (P<0.05) in the percent of motility sperms and degree of grade activity in all taken infertile men while there was significant increase (P<0.05) in the percent of abnormal morphology of sperm in oligospermia and asthenospermia, while there was significant increase (P<0.05) in the mean of while blood cells in all taken infertile men as compared with control group while blood groups there was up of percent in asthenospermia and digospermia in O group, while up of percent in azoospermia there was I A group, while there was up of percent to fertile men in B group.

While the relationships between hormones and other parameters could be summarized as follows:

There was no relationship between the levels of LH hormone and concentration of sperms and grade activity in asthenospermia while there was significant relationship (P<0.05) between the levels of LH hormone and percent of sperms motility and there was no significant relationship (P>0.05) between levels of LH hormone and percent of abnormal morphology of sperm, while there was significant relationship between the levels of LH hormone and the mean of white blood cells in all taken infertile men.

While oligospermia there was significant relationship between the levels of LH hormone and concentration of sperms and the degree of grade activity, while there was no significant relationship between the levels of LH hormone and the percent of sperms motility, while there was no significant relationship between the levels of hormone and the percent of abnormal sperms.

There was no significant relationship between the levels of prolactine hormone and concentration of spermis in asthenospermia and oligospermia, while there was significant relationship between the levels of of hormone and the percent of sperms motility in asthenospermia and oligospermia and there was no significant relationship between the levels of hormone and the degree of grade activity in asthenospermia and oligospermia, while the relationship was no significant between the levels of hormone and percent of abnormal sperms in asthenosperms while there was no significant relationship between the levels of hormone and the number of white blood cells in asthenospermia and azoospermia, while oligospermia there was no relationship.

While  there was no relationship between the levels of testosterone and concentration of sperms in asthenospermia, while there was significant relationship in oligospermia , there was significant relationship between the levels of hormone and the percent of motility sperms and the degree of agrsde activity in asthenospermia and oligospermia, while there was no significant relationship between the levels of hormone and percent of abnormal sperms, there was significant relationship in oligospermia, while there was significant relationship between the levels of hormone and the mean of white blood cells in asthenospermia and oligospermia, while there was no significant relationship between it and the levels of hormone in azoospermia.