دراسة في واسمات مكررات المترادفة القصيرة الجسمية STR في محافظة كربلاء

Study of Autosomal STR in Kerbala Governorate

Short Tandem Repeat analysis is currently the most used method for Treatment forensic evidence, such as linking a suspect to a crime scene, solving problems related to biological relationships, and identifying disaster victims. Some non-coded regions of DNA show significant differences in the basic pair sequence between individuals. Essentially DNA checks involve two steps. Obtain DNA profiles from biological samples and interpret evidence provided from these DNA profiles. DNA was extracted from bone and blood samples, and DNA was estimated and evaluated using statistical interpretation such as probability ratio and parenting index to assess biological evidence. The study included (82) samples, including (50) bone samples, (16) blood samples for fathers, and (16) blood samples for mothers for the purpose of matching. They were distributed in )16( cases and analyzes were performed on the extraction, quantification, DNA amplification, and analysis of data of short tandem repeat data for 24 loci which include Amylogen, D3S1358, D1S1656, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1. Penta D TH01, vWA, D21S11, D7S820, D5S818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, D22S1045 in the Forensic Evidence Investigation Directorate of the Iraqi Ministry of Interior, where the study period was (7) Months since the samples were collected. Through the statistical analyzes in the GenoProof 3 program that we conducted on the genetic features of the DNA of the son, father and mother for 24 Short Tandem Repeat loci and five Short Tandem Repeat loci, we note that in the first case a complete match was obtained for the son’s samples with the father and mother samples, which gave results of more than )99.9%(, Summary 85 which are Acceptable for forensic laboratory and International Society for Forensic Genetics. The probabilities obtained in the second case for 5 Short Tandem Repeat locus for most of the samples were statistically less than the recommendation value (99.9%) forensic laboratory and International Society for Forensic Genetics. Therefore, the second method was used by calculating the combined parenting index and the results were as follows (9.99473712%, 99.99990909 % 99.99846156% 99.99750006% ,99.92313605%, 99.99545475% ,99.99841272%, All results using the above paternity index method were acceptable for the forensic laboratory and International Society for Forensic Genetics.