دراسة مقارنة لتأثير بعض العقاقير والمستخلص المائي لنبات العاقول في طفيلي Entamoeba histolytica وبكتريا السالمونيلا typhi Salmonella داخل الجسم الحي

Comparative study of druges and water plant extracts Alhagi maurorum in Entamoeba histolytica and Salmonella typhi in vivo

This study was conducted in the laboratories of Karbala University – Faculty of Education / Department of Biology and confined for the period between February 2010 to October 2010.
The study examined current and one of the most important parasites and protozoa that infect humans of all ages are Entamoeba histolytica, as it was isolated parasite Children’s Hospital in the province of Karbala by the patients were not subjected to treatment after, and then samples were taken from the feces of infected people has been the diagnosis of the roads common diagnosis (macroscopic, microscopic).
This study also addressed the following:

• Study phenotypic characteristics of the parasite, classification, prevalence, life cycle, methods of transmission of the parasite, pathological effects, symptoms, methods of diagnosis.
• How to deal with the disease (amoebic dysentery) antibiotics such as Amoxicillin and Nalidixic acid and used as substitutes for Metronidazol which represents the factor of control and three levels (250,500,1000) mg / kg, which showed that the Amoxicillin and concentration of 1000 mg / kg was the most effective in Elimination of the parasite after the first four days of dosing, while Nalidixic acid in 5-10 days.
• The study Acol effect of plant extract of the roots of Acol and effectiveness of medical parasite Entamoeba histolytica which could eliminate the parasite after the four days when you use a concentration of 1000 mg / kg.
• the effect of Amoxicillin and Nalidixic acid on each of the liver, kidney, spleen, and through the study of tissue sections for members of the above it is clear that there is no clear effects on the liver, kidney, spleen, and this also applies for the extract of the roots of Acol.
• The sensitivity of bacteria Salmonella causing diarrhea , to different the concentrations of Alhagi extracts inhibition circuit (mm) it was observed that aqueous extract mixture )Alhagi) with an inhibiting effect is evident in these bacteria .
• of DNA extraction of the parasite from infected human feces, feces of infected mouse, from the feces of the mouse through the
• Migration was electric electrophoreses of DNA extracted and then know the location of the gene for grainin bands the parasite in infected humans, 520bp, in infected mouse 400bp ,and intreated mouse           .