دراسة مناعية وتشخيصية لمسببات خمج الأذن الوسطى والتأثير التثبيطي لبعض المستخلصات النباتية والمضادات الحياتية لها

Immunological and Diagnostic Study of the Causes of Otitis Media Infection and the Inhibitory Effect of Some Plant extracts and antibiotic

abstract

Summary
Otitis media is a middle ear infection that can damage the structures around the ear as well as the auditory system. Due to the anatomical structure and growing immune system, it is one of the most prevalent diseases afflicting young children every year.
During the period three months which extending between March 2022 to May 2022 ,ear swabs and blood samples were collected from 101 otitis media patients and 49 healthy control, their ages was around 1 year to 70 years. Collection of samples carried out in two places (Imam Al-Hussein medical city and private clinic). The bacterial growth was identified by using Vitek -2 system. The human Interleukin-12, Intrleukin-13 and Macrophage Inflammatory Protein -1α were measured by using the ELISA technique .The results showed that infection of males(50.5%) was higher than females(49.5%).On the other hand, the highest rate of infection was 21-30 age group while the lowest age was 61-70 years. The results showed appositive association between respiratory tract infection, recurrent otitis media with otitis media infection while the relation was negative with smoking.
Out of 101 ear swabs75 samples (74.25%) had positive bacterial growth, while 26(25.75%) showed negative growth. The results of bacterial isolates were Proteus Mirabilis (17/75) 22.66%, Staphylococcus aureus (16/75) 21.31% , Pseduomonas aeruginosa (8)10.66%, Staphylococcus warneri (6)8%, Aeromonas sobria (5)6.66%.
There were significant differences at (P≤0.05) in serum levels of WBC count between patients ( 9.31mm3) and control ( 7.52mm3).Furthermore ,Lymphocyte count was significant at (P≤0.05)and Granulocyte was significant too at(P≤ 0.025).The interleukins levels were significant also between patients and controls. IL-12 for levels for patients (33.69pg/mL) and in controls ( 20.55pg/mL).Also, there were significant differences at (P≤ 0.05) in the serum levels of IL-13 in patients (48.11pg/mL) while in controls (30.23pg/mL).On the other hand, there were significant differences in MIP-1α at (P≤0.05) the level in patients (168.48pg/mL) while in controls (106.26pg/mL).
Also there were significant differences between Interleukins level and bacterial isolates at (P≤0.05) where the higher level of IL-12 was associated with Staph. aureus infection (26.6±3.8pg/mL), the higher levels of IL-13 was associated with the bacteria Aeromonas sobri (37.9±5 pg/mL) while MIP-1α higher concentration was record also with Staph. aureus bacteria (138.6±11pg/mL).
The antibiotic susceptibility for Staph.aureus isolates were highly sensitive for (amoxillin/clavonic acid 30mg,ceftazimide 30mg,meropenem 10mg, ciprofloxacin 15mg, amikacin 10mg ).
While the bacteria P. mirabilis was highly sensitive for ( ciprofloxacin 10 mg, gentamycin 10 mg).
The gas chromatography mass spectrophotometry for olive and bitter almond oil showed the presence of different biologically active compounds such as (DPPH% ,Total peroxide ,Vitamin C ,Vitamin E, FRAP ,CUPRAC, thiol ,Phenols ,Flavonoids ) the levels of these compound in olive oil was (30.8796 ,13.66667, 1.88 , 2.686, 6.146789, 18.26605, 31.10294, 49, 9.80) respectively.
While the almond oil showed (6.354009, 8.066667, 1.75, 1.75, 16.6055 ,6.761468 ,49.26471, 49.71, 9.9428) respectively.
The stock oil of both (olive oil and bitter Almond oil) showed significant differences at P≤ 0.05 against the growth of S. aureus bacteria, however P. mirabilis had been resistance for the both oils.