دراسة وبائية, مناعية وجزيئية للكشف عن الاصابة بداء المقوسات الكوندية في النساء المجهضات في مدينة كربلاء

"An Epidemiological, Immunological and Moleculare Study to Detect infections with Toxoplasmosis among aborted women in Holy kerbala city"

The current study was designed to investigate infection with the parasite Toxoplasma gondii and evaluate the role of some immune indicators such as cytokinesis IL-1β, cytokinesis IL-10, tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), and molecular detection, which included some mutations in some genes. Including the anti-inflammatory cytokines IL-10 rs1800872 rs1800871 and the proinflammatory IL-1β cytokines rs19644 rs1143627 for patients aborted with toxoplasmosis and patients aborted for other reasons compared with a group of control women. 240 blood samples were collected for patients who aborted with toxoplasmosis and those who aborted for other reasons during the first trimester of pregnancy at the Obstetrics and Gynecology Teaching Hospital in the city of Karbala and during the first of July (2022) until the twenty-second of February (2023).

The required information was recorded according to a special questionnaire form. Prepared in advance for this purpose, the results of the Rapid Test showed that out of 240 aborted women, 60 of them were infected with toxoplasmosis, a percentage of 28.5%, while 150 women who aborted for other reasons had a miscarriage, with a percentage of 71.4% being seronegative. The results of the standard enzyme immunoassay (ELISA) test also showed 60 samples from patients infected with toxoplasmosis, and the total infection rate was 100%, 44 samples were positive for the IgG antibody, at a rate of 73.3%, 7 samples were positive for the IgG antibody, at a rate of 11.6%, and 9 samples were positive for both IgM and IgG, at a rate of 15%, compared with the control group ( Healthy women not infected with the parasite) The immune indicators were also included using the ELISA technique for 60 positive samples, 150 negative samples, and 30 control samples.

The results of the examination of the immune indicators showed that there was a significant increase in the concentrations of cytokinetic TNF-α in patients aborted with toxoplasmosis (31.18±3.4) compared with the mean and standard error. ( 8.45±1.8) and (4.18±0.83 )for both patients who miscarried for other reasons and control women The study also recorded an increase in cytokinetic IL-10 levels at a rate of ( 59.32±3.32) pg/ml, which is significantly higher and at a significance level of >0.05 compared to the average and standard error(29.29±6.01) and (5.08±0.91) pg/ml for each. Of patients who aborted for other reasons and control women While the cytokinesis of IL-1β was significantly increased in patients who aborted with toxoplasmosis at a rate of (24.38±4.21)pg/ml compared to patients who aborted for other reasons and control women (19.47±3.8) and (4.55±1.57)respectively, and the results did not record any significant differences.

The level of TGF-β cytokines in patients aborted with toxoplasmosis and patients aborted for other reasons. An average of, (27.18±4.63) and (25.29±2.6) respectively, compared to the control group, where an average( 4.66±0.78) was recorded. The results of mutation examination for some genotypes of genetic polymorphisms, which included IL-1β cytokines rs1143627 and rs16944 and IL-10 cytokines rs1800872 rs1800871, showed an increase in cytokinesis IL-1β rs16944 at a concentration of (7.63±26.47) pg/ml in patients with aborted patients with toxoplasmosis. gondii women who carry the hybrid genotype TC and CT more than the other alleles TT and CC, and a significant increase of P<0.05 is observed compared to the group of control women (0.42±4.14) pg/ml. On the other hand, the results of the current study showed cytokinetic IL-β for the rs1143627 genotype with a mean and standard deviation of (3.89±20.69) pg/ml in patients of aborted patients with Toxoplasma gondii disease who carry the CC, TT genotype, compared with the other alleles represented by the TC allele and the CT allele, while The group of control women recorded (0.46±2.67) pg/ml. The results of the current study also revealed that cytokinetic IL-10 for the gene rs1800871 recorded a significant increase in both AC and TC alleles, reaching (10.04±61.8) pg/ml for patients aborted with toxoplasmosis and (0.13±3.29) picogram/ml for the group of control women. The results of the current study also recorded a significant increase in the level of cytokinetic IL-10 in the mutant CC allele, with a significant increase of (9.81±47.06) for patients who aborted with toxoplasmosis, while the group of control women recorded (0.91±6.75) Exactly the same results were shown with cytokinetic IL-10 rs1800872. The percentage of cytokinetic IL-10 levels was higher in aborted patients with Toxoplasma gondii infection compared to the control group. While in the current study we did not find the dominant TT allele in both genotype rs1800871 and rs1800872 for patients aborted with toxoplasmosis and control women.